IGEM:Brown/2008/Notebook/Team Resistance/2008/06/08

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Team Resistance Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution. Considerations for gene cassette Harvard’s paper • Used luria lb • Grew cells up to mid-logarithmic stage then added the arabinose • Saw decrease in cell density after just 1 hour, but no change in the control • Used plasmid pvj4 • E.coli strains: DH5alpha Beta2155 • Can we check tightness of promoters by using gfp? Materials for lab: More 1000ml wheaton bottles to be able to store lb (possibly borrow) Requested meeting with professor palmore Questions • What kind of things affect resistance (in terms of our apparatus) wires slipped through cardboard • distance between each wire in plates 0.25 in. and 1.73 in. into plates