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We have 4 tubes from last year. These strains are frozen in glycerol.
- PNZ8901 plasmid in E.coli MC1061 Strain
- Chloroamphenicol resistant
- "sure" vector
- does not integrate
- B. subtilis strain 1A1
- No resistance
- Same as strain 168 but tryptophan deficient
- E.coli strain MC1061, no plasmid
- PP182 plasmid in E.coli strain DH5alpha
- Ampicillin resistance
- integrates
Checking antibiotic resistance
Purpose : Grow each of them on a plate to test their antibiotic resistance
Protocol
- Warm up frozen tubes
- Take 15uL of each and put it on plates without antibiotic
- Incubate at 37°C
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