IGEM:Cambridge/2008/Notebook/Bacillus/2008/07/23
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1/ Purpose Check antibiotic resistance of our different strains
- Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB - Incubate at 37°C 3/ Purpose : Grow plasmids in TOP10, transformation 4 plasmids :
1 control : PUC19 - Add 20uL of TOP10 competent cells and 0.5uL of Plasmid in an eppendorf - 30min on ice - Heat shock : 60s at 42°C - 2min on ice - Add 89.5uL of SOC - 60min at 37°C - Put the mix on plate with ampicillin resistance - Incubate at 37°C
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