IGEM:IMPERIAL/2007/Week5

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Contents

For the Weekend

This Week's Aims

  • Wet lab: Cheuk, Maira, Ben
    • Cloning:
      1. sort out DNA constructs to build
      2. transform cells
      3. amplify biobricks
      4. midi/maxi preps
      5. digestion and ligation
      6. build complete DNA constructs
    • In vitro:
      1. wait for materials/look for BL21
      2. prepare cell extracts


By end of week: Build complete DNA constructs


  • Merge protocols: James, Peixuan, Lucas
    • Define standard units
    • Learn chassis
    • Learn cell-by-date
    • Learn biofilm
      1. merege protocols (prioritise)
      2. provisional lab schedule
      3. learn biofilm production


By end of week: Efficient planning pf protocols and lab sessions


  • Learn vessicles: Dirk, Cheuk
    • Read up papers
      1. materials
      2. equipment
      3. compare results of different techniques
    • Contact people
      1. focus on techniques
      2. ask for opinion


By end of this week: Finalise protocols, Wait for material delivery


  • Wiki: Everyone
    • Have a schedule
      1. weekly
      2. daily
    • Updated project page
      1. progress throughout each day
      2. progress throughout each week
    • Have an iGEM 'digest' for the professors and supervisors (email)


By end of week: Get supervisors back into the 'loop'. Get organised


  • Modelling: Jerry, Alex
    • Learn biofilm
      1. diffusion process within biofilms
      2. [AHL] and temperature dependence
    • Learn cell-by-date
      1. temperature dependence
      2. degradation time
      3. steady state (rate of formation/decay of GFP)


By end of week: Model basic constructs and parameters to be measured

Weekly Outlook

Name Monday Tuesday Wednesday Thursday Friday Saturday Sunday
Alex Define Std Unit Modelling Modelling Modelling Modelling
Anthony Define Std Unit Modelling Modelling Modelling Modelling
Ben Prep/Cloning Wet Lab Wet Lab Wet Lab Wet Lab
Cheuk Definition/Logo Wet Lab /
Learn Vesicles
Wet Lab Wet Lab Wet Lab
Dirk Learn Vesicles Learn Vesicles Learn Vesicles Learn Vesicles Learn Vesicles
James Define Std Unit Merging Protocols Merging Protocols Merging Protocols Merging Protocols
Jerry Modelling Modelling Modelling Modelling Modelling
Lucas Prep/Cloning Merging Protocols Merging Protocols Merging Protocols Merging Protocols
Maira Prep/Cloning Wet Lab Wet Lab Wet Lab Wet Lab
Peixuan Define Std Unit Merging Protocols Merging Protocols Merging Protocols Merging Protocols

Group Timetable

Day Monday Tuesday Wednesday Thursday Friday Saturday Sunday
0900 Daily
Intro
Daily
Intro
Daily
Intro
Daily
Intro
Miniprep Lab Session
0930 Miniprep Lab Session
1000 Miniprep Lab Session
1030 Miniprep Lab Session
1100 Miniprep Lab Session
1130 Miniprep Lab Session
1200 Lunch Lunch Lunch Lunch Lunch
1230 = = = = =
1300 Lab Intro by Ben
1330 Lab Intro by Ben
1400 Meeting with Prof. Freemont
1430 =
1500 =
1530
1600
1630
1700 Debriefing Debriefing Debriefing Debriefing Debriefing
1730 Wikification Wikification Wikification Wikification Wikification
1800
Evening

Expenses

Name Expenditure Description
Total: £0.00


 
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