IGEM:Stanford/2009/Project Homeostasis/Primer/Primersdoc
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| Project |
| Research Proposal |
| Systems Overview |
| Cloning Plan |
| Sequences & Primers |
| Anti-Inflammation |
| Device Overview |
| Parts Design |
| Challenges |
| Results |
| Anti-Immunosuppression |
| Device Overview |
| Parts Design |
| Challenges |
| Results |
| Protocols |
| Modeling |
| Overview |
| Notebook |
| Results |
| Future Work |
| Archived Work |
Primers Compilation
Device 1:
SoxR (Escherichia coli E24377A)
Forward: TATAgaattcgcggccgcttctagATGGAAAAGAAATTACCCCGC
Reverse: TATAtactagtagcggccgctgcagTTAGTTTTGTTCATCTTCCAGCAAGCGTG
SoxS promoter (Created by Joe 7-6-09)
Forward: TATAgaattcgcggccgcttctagagAATCGCTTTACCTCAAGTTAACTTG
Reverse: TATAtactagtagcggccgctgcagCAGTTCGTTAATTCATCTGTTGGG
Blh (uncultured marine bacterium 66A03)
Forward: TATAgaattcgcggccgcttctagATGGGTCTGATGCTGATTG
Reverse: TATAtactagtagcggccgctgcagTCAGTTTTTGATTTTGATACGGG
RalDH(II) (Rattus Norvegicus*)
Forward: TATAgaattcgcggccgcttctagATGCCCGGCGAGGTGAAGGC
Reverse: TATAtactagtagcggccgctgcagTTAGGAGTTCTTCTGGGGGATC
Device 2:
New IL-6 fusion gene (Created by Joe 7-6-09)
Forward: TATAgaattcgcggccgcttctagATGCCAACAATAACCACTGC
Reverse: TATAtactagtagcggccgctgcagTTATGCTGATGCTGTCAAAG
HlyC (from E. coli str. CFT073)
Forward: TATAgaattcgcggccgcttctagagTTGGTTTGCTTTTTTTTACCTGCCACC
Reverse: TATAtactagtagcggccgctgcagCTCTTAACCTGTTAATG
HlyB (from E. coli str. CFT073)
Forward: TATAgaattcgcggccgcttctagagGGAGTCATAATGGATTCTTGTC
Reverse: TATAtactagtagcggccgctgcagGTTAGTCTGACTGTAACTG
HlyD (from E. coli str. CFT073)
Forward: TATAgaattcgcggccgcttctagATGAAAACATGGTTAATGGGGTTCAGCG
Reverse: TATAtactagtagcggccgctgcagCTCTGAGACTTAACGCTCATG
TolC (from E. coli str. CFT073)
Forward: TATAgaattcgcggccgcttctagagGCAAATGAAGAAATTGCTCC
Reverse: TATAtactagtagcggccgctgcagCGTCATCAGTTACGGAAAGGG
TrpR (E. coli str. IAI1)
Forward: TATAgaattcgcggccgcttctagATGGCCCAACAATCACCCTATTCAGC
Reverse: TATAtactagtagcggccgctgcagTCAATCGCTTTTCAGCAACACCTCTTCCAGCCACTGGC
Trp Promoter and Operator (E. coli: no specified strain )
Forward: TATAgaattcgcggccgcttctagagCTCAAGGCGCACTCCCGTTCTG
Reverse: TATAtactagtagcggccgctgcagTCGATACCCTTTTTACGTGAACTTGCGTACTAG
HlyR: Since HlyR is not a protein. This sequence is sequenced as a chunk with HlyA,HlyB, HlyC. Thus only one primer is needed. primer:
TATAtactagtagcggccgctgcagGAATTCCAAGCGAAGTCC
