IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/07/05
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 Bacterial eyespot
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Day 3The two plates 5.A and 5.B gave a good amount of colonies but 100μL is too much to inoculate We picked single colonies and put them in 10 ml LB with Ampicilin 100mg/mL And put the tube to grow at 30°C overnight The tube cultures seem to have gone well but again 1.E is slightly clearer than the others We precipitated tree 2mL tubes for each Biobrick by centrifugation 3min at 13000rpm We then made a miniprep for one of the tubes and froze the two tubes left We used a QIAprep® Spin Miniprep kit from QIAGEN® [1]
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