Keck Subbasement:References

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Protocols

• Accuprime PCR Protocol
• T4 DNA Ligase
• Quick Ligase for the molar ratio calculation; the ligase we have in lab is the T4 DNA ligase (protocol above)
• XL1-Blue Competent Cells
• Current Protocols
• Designing primers
• Fusion PCR Intro

Websites

• PubMed for looking up papers
• NetPrimer primer design tool
• Caltech BioControl Reading Group papers on biological control systems
• APh 162 web site - lots of tutorials and protocols
• primer design
• Basics on Fluorescent Proteins
• Genotypes of E.coli strains
• Double Digest check
• Omega optical
• IDT login

Safety

• MSDS chemical database

Pictures

• 1 kb NEB ladder

• 100 bp NEB ladder

Manuals

• Sony DSC-F707 Cybershot camera (4M PDF)
• Olympus IX71 Inverted Microscope
  • Krato (small room) - Motorized stage, X-Cite 120PC illumination source, Hamamatsu camera. Schedule via Google Calendar (send e-mail to RMM to get added)
  • Nemesis (dark room 1) - Motorized stage, Lumen 200 illuminiation source, Hamamatsu camera, envrionmental chamber. Schedule via Google Calendar (send e-mail to RMM to get added)
  • Kinesin? (on cart) - this microscope showed up in the lab one day. Not sure where it came from. It has a manual stage, X-Cite 120 illumination system and a QImaging camera.
  • Prior ProScan II Motorized Stage
  • Lumen 200 Fluorescence Illumination System
  • X-Cite 120 PC illumination source
  • MicroManager 1.x
• Thermo Genesis 10uv spectrophotometer - spec haven't found a user manual yet
• Techne TC-312 thermal cycler