Klapperich Lab:Notebook/Lab Meeting Notes/2009/03/05

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5 March 2009 Lab Meeting Agenda

  • Status: compressed air and vacuum. DONE

Bioanalyzer DONE
Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look

  • Co. says that it works fine. Transducer up to 1000 psi. We are getting it back.

† Announcements

  • MicroTAS abstracts.


† Flu R01

  • Talked to Patty and Jim today. Need more sample. No more splitting. Compare notes with Sonali, Patty and Jim

  • 3/13 Cathie meeting with Nira Pollcak - more samples
  • BUMC _ John Connor - Cathie working on IBC amendment. Two transportation steps


† SEPSIS Project

  • Submitted to Integrative Biology.


†RNA project


  • New proposal with Fraunhofer for food due this week.


† COBRA

  • Integration test from here forward.
  • Test ongoing with Rubber adhesive tape for Sol-gel.
  • Test with engineered SERS substrates

- Discussion for different approach using COP film as their substrate

  • Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann.
    • benchtop or floor models searched, price range 32K+, waiting on quote.


  • Substrates (JZ)

- Try glass fixture.
- magnets hold Teflon substrate in place and make sure they sink
- they crack as soon as left dry
- nanopatterned COP provides access to repeatable morphology *try EBEAM gold on COP
- Engineered COP substrate?

  • three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD)


  • Colloid experiment.

- Do Rhodamine.

    • ordered

-Need a size specific positive control. PS beads? charged?

    • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    • co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles


† Valve Array/Valve building - done.


† Fraunhofer: LOAC

  • Paper drafting!


† Biointerfaces group


  • Final experiments were done.
  • counting the number of trapped cells
  • adding experimental results in the draft paper.


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • Cathie in charge of PCR1 paper right now.
  • Developed a new protocal for PCR coating
  • Simulaons of DNA chain model are running (60 beads).


† RCA/HDA

  • Cathie submitted One pager Whitepaper. Waiting for response.
  • convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested?
  • PReliminary no-valve design for 25 microliters.
  • 20 more test chips to Sonali (AACC poster).
  • Sonali try to do 10 microliters in the tube


† Senior project

  • Megan
  • Test on Thu, Fri worked but failure after some cycles.
  • Need to enhance bonding (e.g. Ar or O2 ashing)


† F31: Cochlea

  • Cell/matrix paper blocking controls. Likely final questions... when is Decorin up/down regulated--- is there an association between fibronectin and actin cytoskeletons during regeneration? Obtained feedback on culture paper.


† Silica Optimization (Lambda):

  • Do full spectra on dirty controls - with PG and Without PG
  • try another color DNA dye.
  • UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html

  • reading about all the different stains to

Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


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