Klapperich Lab:Notebook/Lab Meeting Notes/2009/03/05

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5 March 2009 Lab Meeting Agenda Status: compressed air and vacuum. DONE Bioanalyzer DONE Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look Co. says that it works fine. Transducer up to 1000 psi. We are getting it back. † Announcements MicroTAS abstracts. † Flu R01 Talked to Patty and Jim today. Need more sample. No more splitting. Compare notes with Sonali, Patty and Jim 3/13 Cathie meeting with Nira Pollcak - more samples BUMC _ John Connor - Cathie working on IBC amendment. Two transportation steps † SEPSIS Project Submitted to Integrative Biology. †RNA project New proposal with Fraunhofer for food due this week. † COBRA Integration test from here forward. Test ongoing with Rubber adhesive tape for Sol-gel. Test with engineered SERS substrates - Discussion for different approach using COP film as their substrate Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann. benchtop or floor models searched, price range 32K+, waiting on quote. Substrates (JZ) - Try glass fixture. - magnets hold Teflon substrate in place and make sure they sink - they crack as soon as left dry - nanopatterned COP provides access to repeatable morphology *try EBEAM gold on COP - Engineered COP substrate? three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD) Colloid experiment. - Do Rhodamine. ordered -Need a size specific positive control. PS beads? charged? 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles † Valve Array/Valve building - done. † Fraunhofer: LOAC Paper drafting! † Biointerfaces group Final experiments were done. counting the number of trapped cells adding experimental results in the draft paper. † CIMIT- Colson Grant IRB still in revision. † PCR Cathie in charge of PCR1 paper right now. Developed a new protocal for PCR coating Simulaons of DNA chain model are running (60 beads). † RCA/HDA Cathie submitted One pager Whitepaper. Waiting for response. convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? PReliminary no-valve design for 25 microliters. 20 more test chips to Sonali (AACC poster). Sonali try to do 10 microliters in the tube † Senior project Megan Test on Thu, Fri worked but failure after some cycles. Need to enhance bonding (e.g. Ar or O2 ashing) † F31: Cochlea Cell/matrix paper blocking controls. Likely final questions... when is Decorin up/down regulated--- is there an association between fibronectin and actin cytoskeletons during regeneration? Obtained feedback on culture paper. † Silica Optimization (Lambda): Do full spectra on dirty controls - with PG and Without PG try another color DNA dye. UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html reading about all the different stains to Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail