Paulsson:Facs

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Where to do it

Systems Biology FACS facility (accessible through SysBio Intranet [1])

talk to Tricia Rogers
No bacteria in the (Aria) sorter!

CBR

requires PO, looser rules, several sorters

Protocols

Thoughts

How to bleach prior to fixation?

test in plate reader with live cells
using scope lamps/lasers?

Post-arrest maturation?

on ice
cholamphenicol
NaN3

Chloride sensitivity (media?)

Biotech and Bioeng v25 no10 p2485

deg-tag vs. photobleach... which ahs better signal strength

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