Where to do it
Systems Biology FACS facility (accessible through SysBio Intranet )
- talk to Tricia Rogers
- No bacteria in the (Aria) sorter!
- requires PO, looser rules, several sorters
How to bleach prior to fixation?
- test in plate reader with live cells
- using scope lamps/lasers?
- on ice
Chloride sensitivity (media?)
Biotech and Bioeng v25 no10 p2485
deg-tag vs. photobleach... which ahs better signal strength