| Name
| Background
| Genotype
| Plasmid(s)
| Note
|
| PMB62
| MC1061
|
| pPM22
| 240 lacO repeats in pSC101 ts-Rep
|
| PMB63
| MC1061
|
| pPM23
| 24 lacO repeats in pSC101 ts-Rep
|
| PMB64
| MC1061
|
| pPM24
| 24 tetO repeats in pSC101 ts-Rep
|
| PMB65
| MC1061
|
| pPM25
| 120 lacO repeats in pSC101 ts-Rep
|
| PMB66
| MC1061
|
| pPM26
| 120 tetO repeats in pSC101 ts-Rep
|
| PMB67
| MC1061
|
| pPM27
| LacI-CFP, TetR-YFP in p15A (CmR, and AmpR!?!), forward
|
| PMB68
| MC1061
|
| pPM28
| LacI-CFP, TetR-YFP in p15A (CmR, and AmpR!?!), reverse
|
| PMB69
| MC1061
|
| pPM29
| Ptet-mCherry in pSC101
|
| PMB70
| MC1061
|
| pPM30
| Plac-mCherry in pSC101
|
| PMB74
| PMB14
|
| pPM29
|
|
| PMB75
| PMB27
|
| pPM30
|
|
| PMB76
| MC1061
|
| pPM31
| LacI-YFP in p15A (CmR, and AmpR!?!)
|
| PMB77
| MC1061
|
| pPM32
| LacI-CFP, TetR-YFP in p15A (CmR only)
|
| PMB78
| DH5α
|
| pPM33
| LacI-YFP in p15A (CmR only)
|
| PMB79
| PMB14
|
| pPM34
|
|
| PMB80
| PMB14
|
| pPM35
|
|
| PMB81
| PMB27
|
| pPM36
|
|
| PMB82
| PMB27
|
| pPM37
|
|
| PMB83
| PMB14
|
| pPM38
| Ptet-mCherry in pSC101 ts-Rep
|
| PMB84
| PMB27
|
| pPM39
| Plac-mCherry in pSC101 ts-Rep
|
| PMB85
| MC1061
|
| pPM40
|
|
| PMB86
| MC1061
|
| pPM41
|
|
| PMB87
| MC1061
|
| pPM42
|
|
| PMB89
| ECF259
|
| pPM32
|
|
| PMB90
| AB1157
|
| pPM32
|
|
| PMB91
| AB1157
|
| pPM33
|
|
| PMB92
| AB1157
|
| pLAU53
|
|
|
| PMB93
| AB1157
|
| pPM32/pPM24
|
|
| PMB94
| AB1157
|
| pPM32/pPM26
|
|
| PMB95
| AB1157
|
| pPM33/pPM22
|
|
| PMB96
| AB1157
|
| pPM33/pPM23
|
|
| PMB97
| AB1157
|
| pPM33/pPM25
|
|
| PMB98
| PMB14
|
| pPM40
|
|
| PMB99
| PMB14
|
| pPM41
|
|
| PMB100
| PMB14
|
| pST26
| same as ST???
|
| PMB101
| PMB14
|
| pST28
| same as ST???
|
| PMB102
| MC4100
| galK::Plac-CFP
|
| Hegreness et al., Science '06, via Kishony Lab
|
| PMB103
| MC4100
| galK::Plac-YFP
|
| Hegreness et al., Science '06, via Kishony Lab
|
| PMB104
| PMB27
| galK::Plac-CFP
|
| PMB102 transduced into PMB27; not confirmed by PCR, only by CFP expression
|
| PMB105
| PMB27
| galK::Plac-YFP
|
| PMB103 transduced into PMB27; not confirmed by PCR, only by YFP expression
|
| PMB106
| PMB104
|
| pST27
| (Plac-Citrus on R1, par-)
|
| PMB107
| PMB104
|
| pPM41
| (Plac-Citrus on mini-F)
|
| PMB108
| PMB105
|
| pST29
| (Plac-mCherry on R1, par-)
|
| PMB110
| PMB27
|
| pST27
| (Plac-Citrus on R1, par-)
|
| PMB111
| PMB27
|
| pST29
| (Plac-mCherry on R1, par-)
|
| PMB112
| PMB104
|
| pST26
| (Ptet-Citrus on R1, par-)
|
| PMB113
| PMB104
|
| pPM40
| (Ptet-Citrus on mini-F)
|
| PMB114
| PMB105
|
| pST28
| (Ptet-mCherry on R1, par-)
|
| PMB115
| PMB105
|
| pPM42
| (Ptet-mCherry on mini-F)
|
| PMB116
| PMB104
|
| pPM40/pST28
| (Ptet-Citrus on mini-F; Ptet-mCherry on R1,par-)
|
| PMB117
| PMB104
|
| pPM40/pST29
| (Ptet-Citrus on mini-F; Plac-mCherry on R1,par-)
|
| PMB118
| PMB107
|
| pPM41/pST28
| (Plac-Citrus on mini-F; Ptet-mCherry on R1,par-)
|
| PMB119
| PMB107
|
| pPM41/pST29
| (Plac-Citrus on mini-F; Plac-mCherry on R1,par-)
|
| PMB120
| PMB14
|
| pPM42
| (Ptet-mCherry on mini-F)
|
| PMB121
| MC1061
|
| pPM43
|
|
| PMB122
| MC1061
|
| pPM44
|
|
| PMB123
| MC1061
|
| pPM45
|
|
| PMB124
| MC1061
|
| pPM46
|
|
| PMB125
| PMB14
|
| pPM43
| (Ptet-YPet on pSC101, ts-Rep)
|
| PMB126
| PMB27
|
| pPM44
| (Plac-YPet on pSC101, ts-Rep)
|
| PMB127
| PMB14
|
| pPM45
| (Ptet-YPet on pSC101)
|
| PMB128
| PMB27
|
| pPM46
| (Plac-YPet on pSC101)
|
| PMB129
| PMB14
|
| pST26/pPM30
|
|
| PMB130
| PMB27
|
| pST26/pPM30
| (Ptet-Citrus on R1, par-; Ptet-mCherry on pSC101)
|
| PMB131
| PMB14
|
| pPM11
|
|
| PMB132
| PMB27
|
| pPM12
|
|
| Name
| Background
| Genotype
| Plasmid(s)
| Note
|
| PMB495
| MC1061
|
| pPM144
| clone 3
|
| PMB496
| MC1061
|
| pPM145
| clone 1
|
| PMB497
| MC1061
|
| pPM146
| clone 1
|
| PMB498
| MC1061
|
| pPM147
| clone 2
|
| PMB499
| MC1061
|
| pPM148
| clone 1
|
| PMB500
| MC1061
|
| pPM149
| clone 1
|
| PMB501
| MC1061
|
| pPM150
| clone 1
|
| PMB502
| MC1061
|
| pPM151
| clone 1
|
| PMB503
| MC1061
|
| pPM152
| clone 1
|
| PMB504
| MC1061
|
| pPM153
| clone 1
|
| PMB505
| MC1061
|
| pPM154
| clone 3
|
| PMB506
| MC1061
|
| pPM155
| clone 1
|
| PMB507
| MC1061
|
| pPM156
| clone 1
|
| PMB508
| MC1061
|
| pPM157
| forthcoming
|
| PMB509
| MC1061
|
| pPM158
| clone 1
|
| PMB510
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM144
|
|
| PMB511
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM148
|
|
| PMB512
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM152
|
|
| PMB513
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM153
|
|
| PMB514
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM156
|
|
| PMB515
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM158
|
|
| PMB516
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM144
|
|
| PMB517
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM148
|
|
| PMB518
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM152
|
|
| PMB519
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM153
|
|
| PMB520
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM156
|
|
| PMB521
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM158
|
|
| PMB522
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM156/pM152
| made from PMB514; selected on LBgluc(0.2%) / carbenicillin(20ug/ml) / Cm(3ug/ml) / Kn(35ug/ml)
|
| PMB523
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM156/pM152
| made from PMB520; selected on LBgluc(0.2%) / carbenicillin(20ug/ml) / Cm(3ug/ml) / Kn(35ug/ml)
|
| PMB524
| MC1061
|
| pPM160
| clone 1
|
| PMB525
| MC1061
|
| pPM161
| clone 1
|
| PMB526
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM160
|
|
| PMB527
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM161
|
|
| PMB528
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM160
|
|
| PMB529
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM161
|
|
| PMB530
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM160/pPM153
|
|
| PMB531
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM161/pPM152
| pSC101_Plac-copA_Kn / R1(-)_Pt7-gfpmut3_Cm / R1(-)_Pt7-mCherry_Ap
|
| PMB532
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM160/pPM153
|
|
| PMB533
| PMB492
| BW27783 araB::T7RNAP_tetA
| pPM54/pPM161/pPM152
|
|
| PMB535
| MC1061
|
| pPM145 A5
| clone A+5; from original transformation, restruck on Ap20; glycerol stock cultured in LB/Ap25
|
| PMB536
| MC1061
|
| pPM145 A6
| clone A+6; from original transformation, restruck on Ap20; glycerol stock cultured in LB/Ap25
|
| PMB537
| MC1061
|
| pPM162
| clone-1; selected on Ap10, restruck on Ap20; glycerol stock cultured in LB/Ap25
|
| PMB538
| MC1061
|
| pPM163
| clone-1; selected on Ap10, restruck on Ap20; glycerol stock cultured in LB/Ap25
|
| PMB539
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM160-1
| redo of PMB526. Selected on Kn35/Cm8, restruck on same; glycerol stock cultured in LB/Cm5 (forgot Kn)
|
| PMB540
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM160-2
| Selected on Kn35/Cm8, restruck on same; glycerol stock cultured in LB/Cm5 (forgot Kn)
|
| PMB541
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM145 (A5)
| selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25
|
| PMB542
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM162-1
| selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25
|
| PMB543
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM163-1(1)
| clone-1, selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25
|
| PMB544
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM163-1(2)
| clone-2(small), selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25
|
| PMB545
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM145 A5-2
| selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20
|
| PMB546
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM163-1a
| selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20; not the same as PMB543=479/163-1(1)
|
| PMB547
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM163-1b
| selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20
|
| PMB548
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM163-1c
| selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20
|
| PMB549
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM145 (A5)
| 145A5 plasmid prep from PMB535 grown in Cb20; trfs selected on Ap10; glycerol stock cultured in LBglucose/Cb10 (forgot Kn)
|
| PMB550
| MC1061
|
| pPM164 (2) - no good
| clone 2; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50
|
| PMB551
| MC1061
|
| pPM164 (3) - no good
| clone 3; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50
|
| PMB552
| MC1061
|
| pPM165 (4)
| clone 4; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50
|
| PMB553
| MC1061
|
| pPM165 (5) - no good
| clone 5; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50
|
| PMB554
| MC1061
|
| pPM166
| clone 25-6; trfs selected on LB/Ap25; glycerol stock cultured in LB/Cb10
|
| PMB555
| MC1061
|
| pPM167
| clone 80-6; trfs selected on LB/Ap80; glycerol stock cultured in LB/Cb10
|
| PMB556
| MC1061
|
| pPM168
| clone 80-7; trfs selected on LB/Ap80; glycerol stock cultured in LB/Cb10
|
| PMB557
| MC1061
|
| pPM169
| clone T1-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
|
| PMB558
| MC1061
|
| pPM170
| clone T2-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
|
| PMB559
| MC1061
|
| pPM171
| clone T3-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
|
| PMB560
| MC1061
|
| pPM172
| clone E1-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
|
| PMB561
| MC1061
|
| pPM173
| clone E2-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
|
| PMB562
| MC1061
|
| pPM174
| clone E3-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
|
| PMB563
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM165
| trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20
|
| PMB564
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM169
| trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20
|
| PMB565
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM170
| trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20
|
| PMB566
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM171
| trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20
|
| PMB567
| MC1061
|
| pPM175
| trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25
|
| PMB568
| MC1061
|
| pPM176
| trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25
|
| PMB569
| MC1061
|
| pPM177
| trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25
|
| PMB570
| MC1061
|
| pPM178
| trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25
|
| PMB571
| MC1061
|
| pPM179
| trfs selected on LB/Ap25; restruck on LB/Ap20; glycerol stock cultured in LB/Ap25
|
| PMB572
| PMB479
| DJ480 araB::T7RNAP_tetA
| pPM54/pPM179
| selected on LBgluc(0.2%)/Kn35/Ap20, grown at 30°C; glycerol stock cultured in LB/Cb25
|
| Name
| Background
| Genotype
| Plasmid(s)
| Note
| Notebook Page
|
| NDL-1
| One Shot 2T1R (Invitrogen)
|
|
| ccdB survival cells. Recovered from pGRG37 by curing.
| L.2.14
|
| NDL-2
| MC4100
| Lac-
| pNDL-17, Alon 2-A-6
|
| e.9.2
|
|
| NDL-3
| MC4100
| Lac-
| pNDL-17, Alon 11-A-6
|
| E.9.2
|
| NDL-4
| MC4100
| Lac-
| pNDL-17, Alon 13-B-9
|
| E.9.2
|
| NDL-5
| MC4100
| Lac-
| pNDL-17, Alon 18-E-2
| E.9.2
|
| NDL-6
| MG1655
|
| pNDL-18
| Promoterless GFP, +CFP, -term
| L.6.14
|
| NDL-7
| MG1655
|
| pNDL-19
| Promoterless GFP,-CFP, +term
| L.6.14
|
| NDL-8
| MG1655
|
| pNDL-20
| promoterless GFP,+term,+CFP
| L.6.14
|
| NDL-9
| MG1655
| attTn7::(no promoter)-GFPmut2
|
| Generated by curing NDL-6 of pNDL-18
| L.6.28
|
| NDL-10
| MG1655
| attTN7::B0015-(no promoter)-GFPmut2
|
| Generated by curing NDL-7 of pNDL-19
| L.6.28
|
| NDL-11
| MG1655
| attTn7::B0015-(no promoter)-GFPmut2
|
| Generated by curing NDL-8 of pNDL-20
| L.6.28
|
| NDL-12
| MG1655
|
| pNDL-21
| Bright ColE1 RNA1-tdCherry on R1
| L.7.19
|
| NDL-13
| MG1655
|
| pNDL-22
| Bright ColE1 RNA1-tdCherry on R1
| L.7.19
|
| NDL-14
| MG1655
|
| pNDL-23
| very bright ColE1 RNA1-tdCherry on R1
| L.7.19
|
| NDL-15
| MG1655
|
| pNDL-24
| very bright ColE1 RNA1-tdCherry on R1
| L.7.19
|
| NDL-16
| MG1655
|
| pNDL-25
| Intermediate bright ColE1 RNA1-tdCherry on R1
| L.7.19
|
| NDL-17
| MG1655
|
| pNDL-26
| Intermediate bright ColE1 RNA1-tdCherry on R1
| L.7.19
|
| NDL-18
| MG1655
|
| pNDL-31
| Bright ColE1 RNA1-tdCherry in pNDL6 integration vector
| L.11.11
|
| NDL-19
| MG1655
|
| pNDL-32
| Very bright ColE1 RNA1-tdCherry in pNDL-6 integration vecotr
| L.11.11
|
| NDL-20
| MG1655
|
| pNDL-33
| Very bright ColE1 RNA1-tdCherry in pNDL-6 integration vector
| L.11.11
|
| NDL-21
| MG1655
| attTn7::pRNA1(bright)-tdCherry
| none
| bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-31)
| L.11.19
|
| NDL-22
| MG1655
| attTn7::pRNA1(very bright)-tdCherry
| none
| Very bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-32)
| L.11.19
|
| NDL-23
| MG1655
| attTn7::pRNA1(very bright)-tdCherry
| none
| Very bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-33)
| L.11.19
|
| NDL-24
| MG1655
|
| pNDL-41
| Contains plasmid with FRT-flanked KanR gene. For Integrations.
|
|
| NDL-25
| DH5-Alpha
| 'WT'
| none
| Stock for cloning strain-- plain DH5 alpha cells.
|
|
| NDL-26
| BL21-AI (Invitrogen)
|
| pNDL-44
| Contains plasmid with T7 His-bt-B-gal expression construct
| 39.25
|
| NDL-27
| MG1655
|
| pNDL-46
| Strain for integration of 1-E-3 Alon Clone
| L.17.1
|
| NLD-28
| MG1655
|
| pNDL-47
| Strain for Integration of 1-E-5 Alon Clone
| L.17.1
|
| NDL-29
| MG1655
|
| pNDL-48
| Strain for Integration of 2-A-6 Alon Clone
| L.17.1
|
| NDL-30
| MG1655
|
| pNDL-49
| Strain for Integration of 2-C-11 Alon Clone
| L.17.1
|
| NDL-31
| MG1655
|
| pNDL-50
| Strain for Integration of 2-F-4 Alon Clone
| L.17.1
|
| NDL-32
| MG1655
|
| none
| NDL-27 cured of pNDL-46. Alon clone integrant.
| L.17.2
|
| NDL-33
| MG1655
|
| none
| NDL-28 cured of pNDL-47. Alon clone integrant.
| L.17.2
|
| NDL-34
| MG1655
|
| none
| NDL-29 cured of pNDL-48. Alon clone integrant.
| L.17.2
|
| NDL-35
| MG1655
|
| none
| NDL-30 cured of pNDL-49. Alon clone integrant.
| L.17.2
|
| NDL-36
| MG1655
|
| none
| NDL-31 cured of pNDL-50. Alon clone integrant.
| L.17.2
|
| NDL-37
| BL-21
| lacY1 mutation (Novagen says its a LacZY mutation)
| none
| LacZ- strain for protein expression. Used in purificaton of btB-gal
| 42.1
|
| NDL-38
| NDL-37 (Novagen Tuner DE3)
| lacY1 mutation (Novagen says its a LacZY mutation)
| pNDL-44
| For purification of btB-gal
| 42.1
|
| NLD-39
| MG1655
| Wanner strain for integration into chromosome
| pKD46 (pNDL-???)
|
|
|
| NDL-40
| MG1655
| pNDL-52 pRNA1-mCherry integrated downstream of attTn7
| cured of pKD46
| Constitutive mCherry strain for library, prior to P1 transduction
| L.18.6
|
| NDL-41
| BL-21 AI
| Contains Ara-Inducible T7 Polymerase
| pNDL-96
| For expression of GST-cotA
| 43.9
|
| NDL-42
| Rosetta2 DE3 (Novagen)
| BL21 derived Strain from Novagen with codon+ plasmid
| pRARE2
| For recombinant protein expression with rare codons
| L.47.1
|
| NDL-43
| MG1655 (CGSC 7740)
| Fully sequenced MG1655 Strain
| none
| Host strain for library construction
| L.18.15
|
| NDL-44
| Tuner BL21 (NDL-37)
| BL21 (DE3) lacZY mutant
| pRARE2 (camR)
| Codon+ Tuner Strain
| ex.48.2
|
| NDL-45
| MG1655
| near attTn7::pBR322 pRNA1-mCherry-FRT-KanR
| None
| Product of P1 from NDL-40 into MG1655 (NDL-43)
| L.18.15
|
| NDL-46
| Tuner BL21 (NDL-37)
| BL21 (DE3) LacZY mutant
| pRARE2 (camR), pNDL-44 (ampR)
| Strain for expression of His-bt-B-gal
| 48.3
|
| NDL-47
| MG1655
| near attTn7::pRNA1-tdCherry
| none
| FLP-removed KanR from NDL-45
| L.18.9
|
| NDL-48
| BW25113
| ΔMotB::KanR
| none
| Keio ΔMotB strain CGSC 9564
| L.19.1
|
| NDL-49
| BW25113
| ΔMotA::KanR
| none
| Keio ΔMotA strain CGSC 9564
| L.19.1
|
| NDL-50
| BW25113
| ΔFliC::KanR
| none
| Keio ΔfliC strain CGSC 9586
| L.19.1
|
| NDL-51
| NDL-47(-3)
| ΔMotB::kanR near attTn7::pRNA1-mCherry
| none
| NDL-47 with keio 9564 deletion
| L.19.5
|
| NDL-52
| NDL-47(-3)
| ΔMotA::kanR near attTn7::pRNA1-mCherry
| none
| NDL-47 with keio 9565 deletion
| L.19.5
|
| NDL-53
| NDL-47(-3)
| ΔfliC::kanR near attTN7::pRNA1-mCherry
| none
| NDL-47 with keio 9586 deletion
| L.19.5
|
| NDL-54
| MG1655
| ΔMotA::kanR
| none (pKD46 cured)
| MG1655 with keio 9565 deletion
| L.20.5
|
| NDL-55
| MG1655
| ΔMotB::kanR
| none (pKD46 cured)
| MG1655 with keio 9564 deletion
| L.20.5
|
| NDL-56
| NDL-47
| ΔMotA::kanR near attTn7::pRNA1-mCherry
| none
| P1vir:NDL-54 -> NDL-47
| L.20.7
|
| NDL-57
| NDL-47
| ΔmotB::kanR near attTn7::pRNA1-mCherry
| none
| p1vir:NDL-55 -> NDL-47
| L.20.7
|
| NDL-58
| NDL-47
| ΔmotA near attTn7::pRNA1-mCherry
| none
| NDL-56 following FLP removal of KanR
| L.20.11
|
| NDL-59
| NDL-47
| ΔmotB near attTn7::pRNA1-mCherry
| none
| NDL-57 following FLP removal of KanR
| L.20.11
|
| NDL-93
| MG1655
| ΔmotA
| none
| NDL-54 following FLP removal of KanR
| L.24.6
|
| NDL-94
| MG1655
| ΔmotB
| none
| NDL-55 following FLP removal of KanR
| L.24.6
|
| NDL-95
| BW25113
| ΔlacI::KanR
| none
| KanR lacI Keio strain
| T.2.1
|
| NDL-96
| MG1655
| ΔlacI::KanR
| none
| P1vir-NDL-95 ---> MG1655
| T.2.1
|
| NDL-97
| MG1655
| DE3 (specR)
| none
| P1vir-PMB14----> MG1655
| T.2.1
|
| NDL-98
| BL21
| DE3
| pNDL-99 (pGK8-GFP003}
|
| T.3.1
|
| NDL-99
| BL21
| DE3
| pNDL-100 (pGK8-GFP008}
|
| T.3.1
|
| NDL-100
| BL21
| DE3
| pNDL-101 (pGK8-GFP012}
|
| T.3.1
|
| NDL-101
| BL21
| DE3
| pNDL-102 (pGK8-GFP021}
|
| T.3.1
|
| NDL-102
| BL21
| DE3
| pNDL-103 (pGK8-GFP037}
|
| T.3.1
|
| NDL-103
| BL21
| DE3
| pNDL-104 (pGK8-GFP038}
|
| T.3.1
|
| NDL-104
| BL21
| DE3
| pNDL-105 (pGK8-GFP053}
|
| T.3.1
|
| NDL-105
| BL21
| DE3
| pNDL-106 (pGK8-GFP100}
|
| T.3.1
|
| NDL-106
| BL21
| DE3
| pNDL-107 (pGK8-GFP170}
|
| T.3.1
|
| NDL-107
| BL21
| DE3
| pNDL-108 (pGK8-GFP182}
|
| T.3.1
|
| NDL-108
| TB10
| Wanner Strain with Heat Inducible Machinery
| none
|
| L.something
|
| NDL-109
| CP78 (CGSC 4695)
| thr,leu,his,arg auxotroph (relA WT)
| none
|
| T.5.1
|
| NDL-110
| CP79 (CGSC 4696)
| thr,leu,his,arg auxotroph (relA2-- null)
| none
|
| T.5.1
|
| NDL-111
| BW25113 (CGSC 10159)
| Keio RelA knockout (kanR)
| none
|
| T.5.1
|
| NDL-153
| CP78 (NDL-109,CGSC 4695)
| thr,leu,his,arg auxotroph (RelA WT)
| pKD46
| Strain for Wanner Integration of GFP Reporters
| T.2.2
|
| NDL-154
| CP79 (NDL-110,CGSC 4696)
| thr,leu,his,arg auxotroph (relA2--null)
| pKD46
| Strain for Wanner Integration of GFP Reporters
| T.2.2
|
| NDL-155
| TB10
| heat inducible labmda red prophage
| none
| pRNA-1/mCherry-FRT-kanR integrant
| L.25.23
|
| NDL-156
| TB10
| heat inducible labmda red prophage
| none
| pRNA-1/mCherry/mKate2 hybrid-FRT-kanR integrant
| L.25.23
|
| NDL-157
| TB10
| heat inducible labmda red prophage
| none
| pRNA-1/mKate2-FRT-kanR integrant
| L.25.23
|
| NDL-158
| NDL-93
| Δ motA
| none
| P1vir_NDL-155--> NDL-93
| L.25.23
|
| NDL-159
| NDL-93
| Δ motA
| none
| P1vir_NDL-156--> NDL-93
| L.25.23
|
| NDL-160
| NDL-93
| Δ motA
| none
| P1vir_NDL-157--> NDL-93
| L.25.23
|
| NDL-161
| NDL-93
| Δ motA near attTN7 pRNA1-mCherry
| none
| FLP removed kanR from NDL-158
| L.25.31
|
| NDL-162
| NDL-93
| Δ motA near attTN7 pRNA1-mCherry-mKate2 hybrid
| none
| FLP removed kanR from NDL-159
| L.25.31
|
| NDL-162
| NDL-93
| Δ motA near attTN7 pRNA1-mKate2
| none
| FLP removed kanR from NDL-160
| L.25.31
|
| NDL-192
| TB10
| Xie lon-Venus fusion
| none
| From Sunney Xie Library
|
| NDL-193
| TB10
| Xie AtpD-Venus fusion
| none
| From Sunney Xie Library
|
| NDL-194
| TB10
| Xie lig-Venus fusion
| none
| From Sunney Xie Library
|
| NDL-195
| TB10
| Xie GapA-Venus fusion
| none
| From Sunney Xie Library
|
| NDL-196
| B. Subtilis
| TMN-690 (pYqxm-mCherry, pHag-GFP)
| none
| from Tom Norman
|
| NDL-197
| B. Subtilis
| TMN-694 (pYqxm-mCherry, pHag-GFP, ΔslrR)
| none
| from Tom Norman
|
| NDL-198
| B. Subtilis
| TMN-
| none
| from Tom Norman
|
| NDL-199
| MG1655 (CGSC 7740)
| WT
| none
| Obtained from CGSC
| L.27.1
|
| NDL-200
| MG1655 (CGSC 6300)
| WT
| none
| Obtained from CGSC (3 colonies saved)
| L.27.1
|
| NDL-201
| MG1655 (CGSC 6300)
| near attTn7::pRNA1-mCherry-mKate2-kanR Hybrid
| none
| Obtained from transduction of NDL-200 with p1Vir.NDL-156
| L.27.2
|
| NDL-202
| MG1655 (CGSC 6300)
| near attTn7::pRNA1-mCherry-mKate2 hybrid
| none
| obtained from NDL-201 by FLP-removal of kanR marker
| L.27.4
|
| NDL-203
| MG1655 (CGSC 6300)
| near attTn7::pRNA1-mCherry-mKate2 hybrid. NDL-54 ΔmotA-kanR
| none
| p1Vir.NDL-54--> NDL-202
| L.27.7
|
| NDL-204
| MG1655 (CGSC 6300)
| near attTn7::pRNA1-mCherry-mKate2 hybrid. NDL-54 ΔmotA
| none
| obtained by FLP removal of kanR marker from NDL-203. NOTE: may still have pCP20. See L.27.8
| L.27.8
|
| NDL-205
| NDL-162
| near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library lon-Venus. See L.29.4 for ID.
| none
| P1vir.NDL-192--> NDL-162
| L.29.2
|
| NDL-206
| NDL-162
| near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library AtpD-Venus. See L.29.4 for ID.
| none
| P1vir.NDL-193--> NDL-162
| L.29.2
|
| NDL-207
| NDL-162
| near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library LigA-VenusSee L.29.4 for ID.
| none
| P1vir.NDL-194--> NDL-162
| L.29.2
|
| NDL-208
| NDL-162
| near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library gapA-Venus. See L.29.4 for ID.
| none
| P1vir.NDL-195--> NDL-162
| L.29.2
|
| NDL-209
| Xie Library strain
| markerless ΔmotA integrated into NDL-192.
| none
| Wanner integrant.
| L.30.4
|
| NDL-210
| Xie Library strain
| markerless ΔmotA integrated into NDL-193.
| none
| Wanner integrant.
| L.30.4
|
| NDL-211
| Xie Library strain
| markerless ΔmotA integrated into NDL-194.
| none
| Wanner integrant.
| L.30.4
|
| NDL-212
| Xie Library strain
| markerless ΔmotA integrated into NDL-195.
| none
| Wanner integrant.
| L.30.4
|
| NDL-237
| BW25113
| CGSC 8590, clpP Keio Knockout
| none
| From CGSC.
| L.34.1
|
| NDL-238
| BW25113
| CGSC 8917, ihfB Keio Knockout
| none
| From CGSC.
| L.34.1
|
| NDL-239
| BW25113
| CGSC 9111, hns Keio Knockout
| none
| From CGSC.
| L.34.1
|
| NDL-240
| BW25113
| CGSC 9441, ihfA Keio Knockout
| none
| From CGSC.
| L.34.1
|
| NDL-241
| BW25113
| CGSC 10954, groL Keio Knockout
| none
| From CGSC.
| L.34.1
|
| NDL-242
| NDL-162
| MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid
| none
| NDL-162 transduced with P1Vir-NDL-237
| L.34.2
|
| NDL-243
| NDL-162
| MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid
| none
| NDL-162 transduced with P1Vir-NDL-238
| L.34.2
|
| NDL-244
| NDL-162
| MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid
| none
| NDL-162 transduced with P1Vir-NDL-239
| L.34.2
|
| NDL-245
| NDL-162
| MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid
| none
| NDL-162 transduced with P1Vir-NDL-240
| L.34.2
|
| NDL-246
| NDL-162
| MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid
| none
| NDL-162 transduced with P1Vir-NDL-241
| L.34.2
|
| NDL-247
| NDL-192
| Xie lon reporter (Xie Background), ΔmotA::kanR
| none
| P1vir.NDL-209 --> NDL192
| L.30.7
|
| NDL-248
| NDL-193
| Xie atpD reporter (Xie Background), ΔmotA::kanR
| none
| P1vir.NDL-210 --> NDL193
| L.30.7
|
| NDL-249
| NDL-194
| Xie lig reporter (Xie Background), ΔmotA::kanR
| none
| P1vir.NDL-211 --> NDL194
| L.30.7
|
| NDL-250
| NDL-195
| Xie gapA reporter (Xie Background), ΔmotA::kanR
| none
| P1vir.NDL-212 --> NDL195
| L.30.7
|
| NDL-254
| TMN1090
| amyE::Pyqxm-CFP (spcR)
| none
| TMN1090 transduced with amyE::Pyqxm-CFP (spcR)
|
| NDL-255
| TMN985
| Δslr::mls
| none
| TMN985 transduced with Δslr::mls
|
| NDL-256
| TMN1049
| TMN1049 Δslr::mls
| none
| TMN1049 transduced with Δslr::mls
|
| NDL-257
| NDL-254 (TMN1090 amyE::Pyqxm-CFP (spcR))
| NDL-254 Δslr::mls
| none
| NDL-254 transduced with Δslr::mls
|
| NDL-258
| NDL-256 (TMN1049 Δslr::mls )
| NDL-256 hagA233V
| none
| NDL-256 transduced with hagA233V (phleo)
|
| NDL-259
| NDL-192
| near attTn7::mCherry-mkate2 hybrid specR
| none
| Wanner integration of constitutive red of NDL-162
| L.31.20
|
| NDL-260
| NDL-194
| near attTn7::mCherry-mkate2 hybrid specR
| none
| Wanner integration of constitutive red of NDL-162
| L.31.20
|
| NDL-261
| NDL-195
| near attTn7::mCherry-mkate2 hybrid specR
| none
| Wanner integration of constitutive red of NDL-162
| L.31.20
|
| NDL-262
| TMN1095
| TMN690 + pSpank-sinI (note: sinI - stop codon)
| none
| Renamed TMN1095.
|
| NDL-263
| TMN1096
| TMN694 + pSpank-sinI (note: sinI - stop codon)
| none
| Renamed TMN1096.
|
| NDL-264
| TMN1101
| TMN694 + > 2x sinI
| none
| Renamed TMN1101.
|
| NDL-265
| TMN1111
| TMN694 + 2x sinI
| none
| Renamed TMN1111.
|
