User contributions for Aida Attar
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23 December 2013
- 18:5418:54, 23 December 2013 diff hist +108 Bitan:todo →In vivo work protocols
- 18:5418:54, 23 December 2013 diff hist +1,791 N Bitan:make subQ pumps New page: Making Subcutaneous pumps at the VA I) Do we have enough of all materials? II) To make 0.9% saline A) 100ml dd H2O, 0.9g NaCl III) to sterilize/filter saline for pump incubation bath ... current
- 18:5118:51, 23 December 2013 diff hist +8,102 N Bitan:mouse sac and tissue collection New page: 1/8/10 Aida Attar Sac and Tissue Collection DAY BEFORE SAC I) Make sac list: mouse id, DOB, diet, experiment start date, sac date, weight, comments II) Get tube racks (# of tubes: 7 or ... current
- 18:4818:48, 23 December 2013 diff hist +921 N Bitan:mouse handling New page: Tanya Liu 4/4/11 Mice handling: 1) Put gloved hand slowly into cage. Let mice sniff/explore for ~15 seconds. 2) Let mouse crawl onto hand a. Can use other hand to guide mouse onto hand ... current
- 18:4518:45, 23 December 2013 diff hist +38 Bitan:todo →In vivo work protocols
- 18:4518:45, 23 December 2013 diff hist +3,264 N Bitan: parafin embedded IHC New page: IMMUNOHISTOCHEMISTRY PROTOCOL FOR PARAFFIN SECTIONS DAY 1 0 start pressure cooker at 450o -fill plastic green slide container with citrate buffer 1 Deparaffinize by placing slides in 4... current
- 18:4318:43, 23 December 2013 diff hist +12,001 N Bitan:western New page: Western Protocol (see bottom for native gels) Day 1 (~3hrs w/o block, 4hrs w/) Transfer and blocking 1. Bring sample in PCR tubes 2. Put blue Novex “Tricine SDS sample buffer 2X” mi... current
- 18:4118:41, 23 December 2013 diff hist +5,234 N Bitan:genotyping New page: Genotyping DNA Extraction 1. Weigh tail samples and confirm they are under 25μg, place in microcentrifuge tube 2. Add 100μL Buffer ATL from QIAamp DNA Mini Kit 3. Add 20μL proteinase... current
- 18:3918:39, 23 December 2013 diff hist +2,108 N Bitan:brain protein extraction New page: Tanya Liu 4/4/11, updated Aida Attar 8-2-12 Brain extractions 1) Make solutions (TBS, TBST, 5 M GuaHCl) and add 2 COMplete protein inhibitor tablets (Roche) + 1 phosphatase inhibitor tab...
- 18:3718:37, 23 December 2013 diff hist +148 Bitan:todo →In vivo work protocols
- 18:3618:36, 23 December 2013 diff hist +2,548 N Bitan:parafin embedded tissue cutting and mounting New page: Cutting (in ImagingCore) 1) gather materials (some which are in top desk drawer to the immediate left of the left microtome): 2 paper towels, 2 razors, a few paintbrushes of different size... current
- 18:2818:28, 23 December 2013 diff hist +34 Bitan:todo →In vivo work protocols
- 18:2818:28, 23 December 2013 diff hist +3,710 N Bitan:frozen section IHC New page: Nov 2013 Aida Attar IHC protocol for frozen slides (3/2/12) From Ed Teng / Fusheng Yang Done by Tanya Liu and Ryan Guglietta and Aida Attar DAY 1: Pretreatment of slides For brains tha... current
- 18:2318:23, 23 December 2013 diff hist +52 Bitan:todo →Experimental Procedures
- 18:1418:14, 23 December 2013 diff hist 0 N File:ImageJ Protocol for IHC aa.doc No edit summary current
- 18:1318:13, 23 December 2013 diff hist +2,755 N Bitan:imageJ using ImageJ for IHC analysis current
12 January 2010
- 16:0416:04, 12 January 2010 diff hist +3,182 N User talk:Aida Attar No edit summary current
- 16:0416:04, 12 January 2010 diff hist +1,118 N User:Aida Attar No edit summary current