Bitan:todo
From OpenWetWare
these are experiments that the wiki does not yet have a protocol for
Contents |
Experimental Procedures
Aβ preparations
- HFIP Film Preparation
- Soluble Oligomers
- Microwave-assisted peptide synthesis
- PICUP
- SATA conjugation to Aβ
Molecular Biology Methods for SELEX
- qPCR
- RNA biotinylation
- PCR Amplification of the Initial ssDNA Library for SELEX experiments
- Agarose gel electrophoresis
- Measuring DNA concentration by absorbence
- In-vitro transcription, labeling and G-50 purification of RNA
- Calculating specific activity of hot CTP after calibration date
- Scintillation counting using the Triathler bench-top counter
- TBE-urea-polyacrylamide gel electrophoresis and autoradiography
- Calculating the yield and specific activity of labeled RNA
- Filter binding for SELEX
- Extraction of RNA from filters
- Autoradiography of hot blots
- Autoradiography of hot gels
Structural Studies
Protein Analysis
- ALISA
- Western Blot
- ImageJ densitometry
- dot blot
- ELISA/ELONA/α-syn-ELISA-Total/α-syn-ELISA-oligo
- Biotinylation of mAb211
Cell Biology
Other amyloidogenic proteins
Aggregation (ThT Fluorescence & EM)
Some Useful Software Usage Tips
other pages we need
Guidelines
Lab Members
Bitan:Research
Publications
Bitan:Talks
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Molecular biology |
Agarose gel electrophoresis |
Biology |
Barnes maze |
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TBE-urea gel electrophoresis |
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Y-maze |
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Autoradiography of hot blots |
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Morris water maze |
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Autoradiography of hot gels |
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Novel object recognition |
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Calculation of RNA and DNA concentration by
absorbance |
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Preparation of tweezers for subcutaneous pumps |
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Calculation of hot RNA specific activity and
yield |
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Oral gavage |
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Desalting of PICUP LMW Aβ40 |
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Paraffin embedding |
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Dot blotting |
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Subcutaneous pump surgery |
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ELISA |
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Immunohistochemistry |
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ELONA |
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In vitro transcription |
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Elution of RNA from columns |
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Intracerebroventricular pump surgery |
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Elution of RNA from filters |
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Intravenous and subcutaneous injection |
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Exonuclease reactions for ssDNA for SELEX |
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Brain protein extraction |
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Filter binding |
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Mouse sacrifice, blood collection, and perfusion |
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Hexokinase reactions |
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Tissue ectioning and mounting |
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Qiagen PCR product purification and measurement
of DNA concentration, agarose electrophoresis |
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Primary rat neurons |
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qPCR/ALISA |
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Dendritic spine morphology |
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Reverse transcription |
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TUNEL staining |
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RNA and DNA labeling |
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MTT |
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RNA extraction, precipitation, and purification |
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LDH |
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Western blot |
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Use of Denville intensifying screens during
autoradiography |
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Scintillation counting using Triathler |
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PCR and use of specific primers for either SELEX
methods, RNA or DNA SELEX |
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SATA conjugation to Aβ |
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Biophysics |
Peptide Synthesis |
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CD |
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PICUP |
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Image analysis (image J) |
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Estimation of methionine sulfoxide
reductase (Msr) activity by HPLC |
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