The Biohydrogen team in the Swartz Lab focuses on developing Biotechnology-based solutions to the global energy problem. This is an important problem: the current method for making hydrogen--steam reformation of natural gas--is energy-intensive and accounts for 2.3% of the total human emissions of carbon dioxide.Specifically, we are interested in developing sustainable processes for the production of hydrogen from (1) Sunlight though an engineered photosynthetic organism and (2) Biomass using engineered cell free extracts.
The Biohydrogen team currently consists of Jamin Koo, Lilian De La Paz, Sylvie Liong, and Marcus Rohovie. Former members of the group include Marcus Boyer, James Stapleton, Jon Kuchenreuter, Phil Smith, Alyssa Bingham, Kunal Mehta, Stacey Shiigi, and Franklin Lu.
Current methods to produce biochemicals rely primarily on existing sources of stored energy such as glucose that can be converted metabolically into useful end products such as ethanol. However, the production of those sugars are driven from complex chemistry within plants that capture energy from the sun through photosynthesis. The primary goal of this research is to engineer a photosynthetic bacteria to produce hydrogen directly from light. Currently, research is focused on both developing new tools for genetic engineering of cyanobacteria, as well as strain engineering for optimal expression of a synthetic pathway to accept electrons from photosynthetic machinery and redirect them to the production of hydrogen. Along these lines we are also interested in protein engineering of heterologous genes in cyanobacteria to efficiently couple with natural systems.
An alternative approach for generation of biohydrogen seeks to capitalize on current research into the efforts to efficiently break down cellulosic biomass including feedstocks such as corn stover which are currently left in fields agricultural residual. The DOE estimates up to a billion tons of material could be available to be converted to useful energy and chemical products. We seek to utilize the sugar units freed from the breakdown of cellulosic biomass which consist primarily of glucose as well as other five carbon sugars such as xylose and convert that to biohydrogen.
In order to maximize the both the yield and minimize the cost, our current research focuses on the generation of cell free extracts that can cheaply and rapidly produce enzymes we need for specific chemical conversions and couple those to a synthetic pathway to generate hydrogen. Consequently we are focused on both development and optimization of a synthetic enzymatic pathway to hydrogen through protein engineering and on cell free metabolic engineering to selectively knockdown and up-regulate required enzymes to maximize yield.
The third major focus of the biohydrogen group is on the shared critical protein for both methods above, an [FeFe] hydrogenase, from the organism Clostridium Pasteurium. Both current and previous students have focused on engineering an oxygen tolerant hydrogenase that has both a high rate of hydrogen formation and resistance to inactivation. These proteins catalyze the interconversion of protons, electrons, and dihydrogen; they are especially oxygen-sensitive due their FeS chemistry.To further these goals we are developing high throughput tools to rapidly screen large libraries for hydrogen production, as well as rationally designing hydrogenase mutations in attempts to confer resistance to oxygen. Simultaneously, we are investigating the complex mechanisms for production and maturation of these enzymes in an effort to better understand the fundamental biochemistry that drives the assembly and activity of these natural hydrogen producers. In this work, we are identifying key components of hydrogenase maturation and in collaboration with the Cramer and Britt labs at UC Davis, are analyzing these changes through EPR and Mossbauer spectroscopy.
Funding: We are grateful for funding from GCEP and the DOE