Talk:Endy:Measuring PoPS on a plate reader

Comments

Equation (5) may not be really practical as it involves [P] and its derivative. However, in this simple synthesis/degradation model, you can show that there is a stable steady state. At this point you will have d[P]/t=0 and your PoPs equation get simplified, so it becomes only function of [D] and [P]. [D] is the number of copies of your promoter (can be measured).And if GFP is used as a reporter, [P] could be measured through a calibrated fluorescent assay.

In Practice, using GFP (45min half life) as a reporter for a promoter characterization could mean that you need to wait 3-4h before steady state. User:Vincent