UA Biophysics:Protocols: Lipid stock

Lipid stock

1. Lipids from the company are stored to -20°C for ensure stability
2. Prior to weighting, lipids should be brought to room temperature in a container containing a dessicant (bag with silica gel). This step reduce the lipid hydration.
3. Weigh the lipid in the analytical balance into glass vials.
4. Add an organic solvent to obtain a stock at 20 mg/ml. Chloroform is used to the most of lipids, but sometimes methanol is need for solubilization of charged lipids. Stock is save at -20°C.

To PG liposomes is recomended use a solution of cloroform:Methanol:dH2O (97:2:1)

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