User:Andrew Perry/Lesser Known Methods in Bacterial Protein Expression
Lesser known methods in recombinant bacterial protein expression
Here are a few lesser known methods / protocols / tricks I've collected over the years. I've love to know if any of them are actually useful for anyone.
Self cleaving TEV protease fusion partner
Thermostable fusion partners
Some elastin-like peptides undergo a soluble–insoluble phase transition ("precipitation"), which can be modulated by salt concentration. When used as a fusion partner, it has be shown that this property can be used as a simple purification step.