User:Andrew Perry/Lesser Known Methods in Bacterial Protein Expression
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Lesser known methods in recombinant bacterial protein expression
Here are a few lesser known methods / protocols / tricks I've collected over the years. I've love to know if any of them are actually useful for anyone.
Self cleaving TEV protease fusion partner
Thermostable fusion partners
Elastin tagging
Some elastin-like peptides undergo a soluble–insoluble phase transition ("precipitation"), which can be modulated by salt concentration. When used as a fusion partner, it has be shown that this property can be used as a simple purification step.
It appears many methods relating to the use of elastin-like peptides may be patent encumbered.
