User:Elaine Marie Robbins/Notebook/CHEM-496/2011/12/06

Properties of AuNP IV

Main project page Previous entry      Next entry

Objective To try to control the aggregation process of AuNP/BSA. In addition, to repeat the experiment performed on 11/30/11. Description Make 4 solutions of AuNP: Solutions 1 and 2 (Au/BSA mole ratio = 70) Combine 0.5 mL of 2.9 mM HAuCl4 and 1.20 mL of 0.0172 mM BSA in a test tube. Add 5 mL of water. Place in oven at 70°C. Solutions 3 and 4 (Au/BSA mole ratio = 166) Combine 0.5 mL of 2.9 mM HAuCl4 and 0.51 mL of 0.0172 mM BSA in a test tube. Add 5 mL of water. Place in oven at 70°C. Leave solutions 1 and 3 in oven for 3.5 hr. Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr. Take UV vis data of solutions 1 and 2. Centrifuge 1 mL of solutions 3 and 4 for 10 min at 13200 rpm. Take UV vis data of the supernatant. Data Solution 1: Au/BSA = 70, left in the oven Solution 2: Au/BSA = 70, taken out of the oven every 30 minutes Solution 3: Au/BSA = 166, left in the oven Solution 4: Au/BSA = 166, taken out of the oven every 30 minutes Notes Results are similar to those observed on 11/30/2011.