User:Floriane Briere/Notebook/CHEM-496/2011/11/02

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Objective Today we are going to perform two different experiments: We are going to perform some Gold NPs again using exactly the same protocol as yesterday (1st of November) but we are going to use a fresh gold solution. We are going to transform the mutate DNA we synthesized yesterday (1st of November) thanks to the PCR technique. Description Gold NPs synthesis: In this specific order, add 1ml of BSA stock solution (15.5µM) + 1ml of HAuCl4 stock solution (2.9mM) + 8ml of water Place in the oven for 2 hours; remove every 30 minutes and leave the solution at room temperature for 10minutes DNA transformation Prepare the LB/agar plate: Add 0.875g LB + 0.7g Agar + 35ml H2O Leave the solution in the autoplate for 1hours and a half Add 35µL of Antibiotics (Ampicillin) + 35mL of water Put some solution into a plastic Petri dish Let the solution become solid Prepare the cells: Place the DNA solution in the ice during Add 5µL of DNA solution to 30µL of cells solution Incubate on ice during 30 minutes Place the solution at 42°C for 30 seconds to make a heat shock Incubate in ice for 5 minutes Add 250μL of SOC media Incubate in shaker at 37°C for 1 hour Results At the end of the Gold NPs synthesis, we obtained some purple fibers; they began to form after 1 hours in the oven and became darker after 1 hours and a half; the fibers are spread through the solution and don't form an aggreggate like yesterday.