User:James C. Schwabacher/Notebook/CHEM-571/2013/10/01

Biomaterials Design Lab

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Objective Monitor the yield and kinetics of HRP-catalyzed oxidation of luminol. Procedure 1. UV-Vis Absorbance of reactants, catalysts, and products horseradish peroxidase (Use stock solution) luminol (use stock solution) 3-aminophthalic acid (product of reaction between luminol and H2O2 catalyzed by HRP. In order to take this measurement, react (in 1:1 ratio or with slight excess H2O2) luminol with H2O2 in presence of HRP. Allow the reaction to proceed for 5 minutes; take the spectrum) Adapted from Dr. Hartings Data Notes