User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/05/21
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May 21st, 2010
1. Re-run gel to verify DNA extraction from E. coli K12 made on may 19th by Augusto & Paz, gel was run 50 min at 90V.
2. I incubated E. coli K12 on 5ml of LB medium for 12 hrs. at 300 rpm, to extract DNA using Miguel’s protocol. I also went to ask Miguel about his protocol but, fortunately, he gave me DNA from E. coli K12, so I don’t have to do DNA extraction again. With the DNA Miguel gave me, I ran a gel to verify the prescence of genomic DNA. In the same gel I loaded Paz’s DNA extraction but now with 5ul of loading buffer. The results are presented in the next figure:
3. PCR for Blue Promoter