User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/19
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March 19, 2013Hybridization of thiol DNA, AuNP, and Molecular BeaconDNA:AuNP ratio 75:1<br.> AuNP concentration: 19.4nM<br.> thiol DNA concentration: 1455nM<br.>
heated for approximately 25 minutes at 70°C; cooled to room temperature<br.>
(7310)V=(800nM)(150μL)<br.> V= 5.5μL MB in 44.5μL filtered H2O<br.> Combine: 50μL 800nM MB, 50μL 727.5nM thiol DNA with 9.7nM AuNP<br.> heated for approximately 25 minutes at 70°C; cooled to room temperature<br.> Procedurethiol DNA and AuNP solution-
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