July 18, 2013
Procedures: Gold Nanoparticle Synthesis by Sodium Citrate Reduction Methods
Desired gold nanoparticle size: approximately 10nm.
Adapted from Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles.
- Boil 50mL MilliQ water in a clean beaker
- Add 0.5 mL of HAuCl4 solution (1% w/v)
- 1% w/v = (0.01g in 1mL water)
- Follow with 1.75 mL of sodium citrate solution (1% w/v)
- Boil for about 10 minutes until solution color changes to bright red
- Replace water lost to evaporation during boiling
- If a higher concentration is desired, centrifuge at 14 000 rpm and resuspended in MilliQ water
Adapted from 4-(Dimethylamino)butyric Acid Labeling for Electrochemiluminescence Detection of Biological Substances by Increasing Sensitivity with Gold Nanoparticle Amplification.
- Bring 100 mL of solution containing 0.01 g of HAuCl4·3H2O to a boil
- Add 3 mL of 1% sodium citrate solution while stirring
- Boil solution for another 40 minutes
- Cool to room temperature
From Allison Alix on 05/10/2013.
- Stir and heat to boiling 20mL of 1mM HAuCl4
- Add 2mL 1% sodium citrate
- Observe a purple to red color change
- Determine size and concentration of AuNP using UV-Vis
Solution too concentrated; will be diluted and measurements taken again tomorrow.