User:Maria Briscione/Notebook/Chem 571/2011/09/20

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Objective

Description

Forward and reverse PCR primers were determined in accordance to the guidelines set forth by the Quick Change Manual. Then a PCR reaction was carried out using previously prepared primers. First, 43 μL of ddH2O was added. Then 1 μL on a 0.5 μ/μL of template DNA, 1 μL of a 10mM dNTP solution, and 1.25 μL of a 100 ng/μL solution of forward and reverse primers was pipetting into the solution. Finally, immediately before the solution was put into a thermocycler 1 μL of enzymes were added to the solution and 25 μL of wax was added slowly to the surface of the solution.

Data

Temperature Cycling:


Primers:

  • FORWARD: 5' GAC GAT GAC GAT AAG GAT CGA TGG GGA TCC GAA 3'
  • REVERSE 5' TTC GGA TCC CCA CCA TCG ATC CTT ATC GTC ATC GTC 3'

Notes

This area is for any observations or conclusions that you would like to note.


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