User:Maria Briscione/Notebook/Chem 571/2011/09/21

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Objective

Description

The PCR products were run using gel electrophoresis. The .01g/mL agarose gel was prepared by combining .25 g of agarose with 25 mL of TAE buffer. The solution was microwaved for 40 seconds and poured into a mold. The solution was left to sit for 15 minutes. Once solidiefied the gel was covered in TAE buffer. The PCR products were then prepared for gel electrophoresis. Five μL of the PCR products and 1 μL of gel loading dye (containing glyercol and dye) were placed on parafilm wax and mixed together. The solution was then loaded into the gel and ran at 80 V for 40 minutes. The gel was then stained by soaking it in a solution of TAE and dilute ethidium bromide and was stirred for 15 minutes. The gel was then washed with a solution containing just TAE.

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