User:Maria Briscione/Notebook/Chem 571/2011/12/07

From OpenWetWare

Jump to: navigation, search
Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

The purpose of the present experiment was to further determine the effect of salt on gold nanoparticle synthesis and "brain formation".

Description

The test tubes were maintained in an 85 degree heat bath for 2.5 hours. A 166:1 ratio of gold to BSA was used for all solutions.

Test tube 1

  • glass test tube
  • .1 ml (1 M) NaCl = 1 mM concentration
  • 1 ml 17.7 µM BSA + 2.34 ml 2.9 mM Au + 6.65 ml water

Test tube 2

  • glass test tube
  • 1 µl(1 M) NaCl = 10 µM concentration
  • 1 ml 17.7 µM BSA + 2.34 ml 2.9 mM Au + 6.659 ml water

Test tube 3

  • plastic test tube
  • .5 ml of .1 ml (1 M) NaCl solution = 1 mM concentration
  • 1 ml 17.7 µM BSA + 2.34 ml 2.9 mM Au + 6.65 ml water

Test tube 4

  • plastic test tube
  • .5 ml of 1 µl(1 M) NaCl solution = 10 µM concentration
  • 1 ml 17.7 µM BSA + 2.34 ml 2.9 mM Au + 6.659 ml water

Data

  • test tube 1 = light yellow fibers
  • test tube 2 = small "brain" on surface with slight purple to it, but mostly yellow fibers throughout
  • test tube 3 = light purple fibers
  • test tube 4 = yellow fibers

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


Personal tools