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Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/30
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Genetically Encoded Memory
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2008
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GammaDelta
Host: Escherichia coli
Classification: serine resolvase
structure:
Length: 183 aa
Amino-Acid-Sequence
Domains
Catalytic site has R8, S10, D67, R68
Li et al 2005 science
Target sequence: two identical simple sequence < 50 bp
res-I, res-II, res-III
Mechanism:
two dimer per site (see diagram from the ref)
Engineering
Mutation
Mutation could change specificity to HK022's site
Chimeric
Directed-Evolution
Exision:
Unnatural-Host
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