User:Sayed Massie Husaini/Notebook/september 5 2017/2017/09/20

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Dialysis Stabilizer exchange: 2, 8cm – 10 cm lengths were cut of 3500 MWCO dialysis tubing & soak in DI water for 10 min. Removed 1, clamped one end, opened other end and transfered exactly 3 mL DI water into tubing. Clamp other end. Place in 125 mL wide mouth jar, add exactly 100 mL 25 mM HdMe2ImCl and screw on lid. Record time. Remove 2nd tubing, clamp end, transfer exactly 3 mL AuNP stabilized with basic AA. Clamp other end. When 15 minutes after recorded time has past, place in 2nd 125 mL jar, add exactly 100 mL 25 mM HdMe2ImCl and screw on lid. Record time. Every 30 minutes, remove exactly 1 mL of solution from jar and measure the absorbance at 211 nm using the spectrophotometer (single wavelength spectrometer). Note that you will be recording a value every 15 minutes. At some point, you will need to measure the absorbance of the HdMe2ImCl stock solution and your AuNP solution. At end of day, cap jar and leave solution until the next session. Other exchanges – perform in between UV measurements Using same procedure to prepare dialysis tubes, prepare a total of 4 dialysis tubes. Dialysis 3 mL AuNP stabilized with basic AA against Glycine buffer Dialysis 3 mL AuNP stabilized with basic AA against water Dialysis 3 mL AuNP stabilized with BSA against Glycine buffer Dialysis 3 mL AuNP stabilized with BSA against water Dialyze until your next session During the next session, you will record the UV (using spectrometer) of solutions inside and outside of each jar. If the UV spectrometer is too busy, you can store portions of the solutions in a centrifuge tube. Dialysis calculations: