User:Sayed Massie Husaini/Notebook/september 5 2017/2017/09/26

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Fluorescence Dialysis excel chart /var/folders/jh/ncy3vnb93nnf0yzqyl4k3hn80000gn/T/com.skitch.skitch/DMD38DAAD84-D9B3-4CB1-9FEE-B247F78DD8A5/Blank.pdf Fluorescence Prepare dilute (1 – 10 μM) solutions of acridine orange and fluorescein. Measure fluorescence of your stock solutions using excitation wavelengths of 308 nm, 440 nm, and 490 nm. You may need to dilute your stock solutions to record peaks below 1000 intensity. Make sure you note the dilutions required. Measure fluorescence of your AuNP using each AA and BSA. Use AuNP formed from the same concentration of HAuCl4 at all three excitation wavelengths. Then, measure the fluorescence of the more concentrated AuNP using the Au:AA = 10:1 Choose any one of the AuNP solutions that had a photoluminescence band around 440 nm Combine with acridine orange (at a concentration equal to one used in step 2) and excite at 308 nm. Measure the fluorescence up to 600 nm. Is the emission band associated with acridine orange visible? Combine with fluorescein (at a concentration equal to one used in step 2) and excite at 490 nm. Measure the fluorescence up to 700 nm. Is the emission band associated with fluorescein quenched? If you have time, try with another AuNP solution that had slightly different fluorescence spectra Spectra: