User:Sayed Massie Husaini/Notebook/september 5 2017/2017/09/27

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AuNP using D2O/H2O UV/VIS Recall that gold absorbs at 265 nm and HxMe2ImCl absorbs at 211 nm Furthermore, the broad absorbance of AuNP at low wavelengths may cause overlaps We will test for the movement of HxMe2ImCl. There probably is too little AuNP in outside chamber to measure. Use a low volume cuvette (semi-micro hold ~ 1 mL and micro hold 0.2 mL) Wash sequentially and dry Wash with water. Use a 200 uL pipette to remove last bits of fluid Wash with 10% HCl (or 1 M). Use a 200 uL pipette to remove last bits of fluid Wash with acetone. Use a 200 uL pipette to remove last bits of fluid Let dry for 5 minutes Run a pure water sample every 3 – 6 measurements to ensure your washing procedure is efficient AuNP using D2O Add 1 mL D2O to your dried sample of HAuCl4 Gently shake to completely dissolve Add 0.1 mL of 1 M NaOH in D2O, between 0.0007 and 0.0009 g of AA, and 0.9 mL D2O Gently shake to completely dissolve Cover with foil AuNP with H2O I have ordered more D2O, which can be used at a later date Prepare 2 mL samples using the following volumes of stock solutions I have prepared samples of 10 mM AuCl3 and 1 M NaOH Sample AA (g) 10mM AuCl3 (mL) 1M NaOH (mL) water (mL) 1 0.004 0.4 0.1 1.5 2 0.0008 0.2 0.1 1.7 3 0.004 0.2 0.1 1.7 4 0.0008 0.4 0.1 1.5 Repeat entire table using your HAuCl4 instead of AuCl3