User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/29

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SDS-PAGE& Finalizing RII overlay Main project page
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Summary

  • Picture RII overlay overnight
  • 2x 8% SD-PAGE Gel
Lane 1 2 3 4 5 6 7 8 9 10
Gel #1
Marker
S0
Lysate (-)
S0
NAC (-)
S0
Beads Sample (-)
x
S0
Lysate
S0
NAC
S0
Beads Sample
x
x
Gel #2
x
(some marker)
Marker
CSE
Lysate (-)
CSE
NAC (-)
CSE
Beads Sample (-)
x
CSE
Lysate
CSE
NAC
CSE
Beads Sample
x

∞ NAC is supernatant left after incubation with beads


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