User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/30

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Splitting cells, Immunoblot Main project page
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Summary

  • Split cells for RII overlay stimulation
  • Split cells for ELISA stimulation?
    • Dependent on how fast Alamar blue gets here
  • Antibody incubation for blot blocked ON
    • mouse anti-RIIα (1:5000) for 2 hours @ RT
    • Wash 3x 10 min. block buffer (1% BSA)
    • anti-mouse secondary antibody (1:10 000) 1 h @ RT
    • Rinse with block buffer
    • Wash 3x 15 min. w. block buffer


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