User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/13

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SDS-PAGE Lysates, strip blot

Summary

Lysate samples were prepared, undiluted samples (75 µL) with NuPage loading buffer + DTT (25 µL)
Samples were put to 70 °C for 10 min.
Samples were put to 4-12% Gradient NuPage gel 200 V

**4-12% Gradient NuPage Gel loading LYSATES hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010**
#	1	2	3	4	5	6	7	8	9	10	11	12
Gel Lysates	X	Invitrogen Marker (8 μL)	Jurkat (20 μL)	HeLa -S (20 μL)	HEK293 (20 μL)	Biorad Marker (8 μL)	S1 (20 μL)	S2 (20 μL)	S3 (20 μL)	C1 (20 μL)	C2 (20 μL)	C3 (20 μL)

Stripping solution
- 50 mL dH₂O
- 5 mL Tris-Cl (1.0 M, pH 6.8) (Stacking gel buffer SDS-PAGE)
- 1 g SDS
- 150 mg DTT (Add later)