Magni:Protocols: Difference between revisions
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*[[Magni:Protocols/tecan|Assays with Tecan Infinite F200 microplate reader]] | |||
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Revision as of 15:21, 30 July 2012
Growth media
Plasmid construction
- Competent cells
- Plasmid resuspension from iGEM kit plates
- Transformation
- Plasmid propagation
- Miniprep
- BioBrick digestion with restriction enzymes
- Electrophoresis
- Gel extraction
- DNA purification (with columns)
- DNA purification via ethanol precipitation with sodium acetate
- Ligation
- PCR
- DNA dephosphorylation with Antarctic Phosphatase
