IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation: Difference between revisions

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==Ligation strategy==
==Ligation strategy==
*[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page See ligation page]
 
 
 
 
<u><font size="2">'''Ligating the Predator Construct:'''</font></u>
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4
|-
!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart></center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center> || ||  ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart></center>|| !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]][[Image:Plus sign.png|20px]][[Image:J37024 logo.png]]</center> || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart></center>||  ||  !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]][[Image:J37024 logo.png]]</center> ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart></center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center> || || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart></center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png]][[Image:C0062 logo.png]]</center> || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37036</bbpart></center>|| ||  || !!style="background:#CCEBAD" | <center>[[Image:J37036 logo.png]]</center>
|}
 
<u><font size="2">'''Ligating the Prey Construct:'''</font></u>
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4
|-
!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart></center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center> || ||  ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart></center>|| !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]][[Image:Plus sign.png|20px]][[Image:J37024 logo.png]]</center> || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart></center>||  ||  !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]][[Image:J37024 logo.png]]</center> ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart></center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center> || || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart></center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png]][[Image:C0062 logo.png]]</center> || ||
|-
!style="background:#D9F2ED"|<center><bbpart>J37036</bbpart></center>|| ||  || !!style="background:#CCEBAD" | <center>[[Image:J37036 logo.png]]</center>
|}


==Quality control==
==Quality control==

Revision as of 00:00, 29 October 2006

Super Parts Not applicable
Actual Part
Sub Parts Prey Construct Predator Construct


Ligation strategy

Ligating the Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
<bbpart>J37024</bbpart>
<bbpart>J37025</bbpart>
<bbpart>J37033</bbpart>
<bbpart>J37019</bbpart>
<bbpart>J37036</bbpart>

Ligating the Prey Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
<bbpart>J37024</bbpart>
<bbpart>J37025</bbpart>
<bbpart>J37033</bbpart>
<bbpart>J37019</bbpart>
<bbpart>J37036</bbpart>

Quality control

Part Logo Built Sequencing Results Primers Used for Sequencing
<bbpart>J37015</bbpart>
Yes sequencing analysis and link to sequence results primers used
<bbpart>J37036</bbpart> No N/A primers used

WetLab work entries

  • The prey has been constructed and tested but is waiting parameter extraction.
  • The predator construct has not been ligated.
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