IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation: Difference between revisions
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::*High signal to noise ratio | ::*High signal to noise ratio | ||
::*Controllable frequency and amplitude | ::*Controllable frequency and amplitude | ||
::*(Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification). | |||
==Enviroment== | ==Enviroment== | ||
Revision as of 02:44, 29 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | 
| |
| Sub Parts | Prey Construct | Predator Construct |
Motivations
- To see whether oscillations, which match are specifications, are produced. We will have fulfilled our specifications if the oscillations have the following properties:
- Stable oscillations for greater than 10 cycles
- High signal to noise ratio
- Controllable frequency and amplitude
- (Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification).
Enviroment
This system will run in a Chemostat. This pice of equipment will keep the cell populations constant and thus keep the pareameters constant throught the course of the experiment. chemostat page
Protocol
- A formal protocol has not been written.
- A suggested method was to aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay
Results
- The predator construct has to be built.
