IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation: Difference between revisions
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::*Stable oscillations for greater than 10 cycles | ::*Stable oscillations for greater than 10 cycles | ||
::*High signal to noise ratio | ::*High signal to noise ratio | ||
::*Controllable frequency | ::*Controllable amplitude and frequency | ||
::*(Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification). | ::*(Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification). | ||
*The testing will be done in a [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation/chemostat|chemostat]]. This equipment works to: | |||
::*Keep the cells in the exponential growth phase | |||
::*Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio | |||
::*Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat | |||
==Protocol== | ==Protocol== | ||
*A formal protocol has not been written. | *A formal protocol has not been written. | ||
* | *Methods for testing under consideration are: | ||
::*To aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay. | |||
::*Use a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Bio_elec_interface biosensor] to measure oscillating output directly (in this case we would be measuring change in pH in the extracellular medium). | |||
::*Use High Performance Liquid Chromatography (more research is required). | |||
==Results== | ==Results== | ||
*The predator construct | |||
*The prey construct has been built but still needs to be characterized | |||
*The predator construct still needs to be built | |||
Revision as of 03:03, 29 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | 
| |
| Sub Parts | Prey Construct | Predator Construct |
Motivations
- To see whether oscillations, which match are specifications, are produced. We will have fulfilled our specifications if the oscillations have the following properties:
- Stable oscillations for greater than 10 cycles
- High signal to noise ratio
- Controllable amplitude and frequency
- (Our system is based on the quorum sensing/quenching system so connectivity is an inherent part of our design. However, more research is to be done to fulfil this specification).
- The testing will be done in a chemostat. This equipment works to:
- Keep the cells in the exponential growth phase
- Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
- Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat
Protocol
- A formal protocol has not been written.
- Methods for testing under consideration are:
- To aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay.
- Use a biosensor to measure oscillating output directly (in this case we would be measuring change in pH in the extracellular medium).
- Use High Performance Liquid Chromatography (more research is required).
Results
- The prey construct has been built but still needs to be characterized
- The predator construct still needs to be built
