Maheshri:Internal
From OpenWetWare
General Lab Policies
- Guidelines for New Members
- Lab Duties
- Standard Operating Procedures for the Equipment
- Common Area Etiquette
- Locations of Common Items and Reagents
- Ordering
- Computing
Protocols
- Media
- Buffers
- Culturing Yeast
- Non-selective Mating of Yeast
- Freezing Cells for Long-term Storage
- PCR
- Restriction Digestion
- Agarose Gel Electrophoresis
- DNA Ligation
- Making Chemically Competent Bacteria
- Bacterial Transformation
- Yeast Transformation
- Bacterial Plasmid Miniprep
- Yeast Genomic Prep
- Yeast Plasmid Prep
- Ethanol Precipitation of Nucleic Acids
- Yeast Protein Prep
- SDS-PAGE
- Western Blotting
- mRNA FISH
- Micrococcal Nuclease Assay
- RT-PCR
- Fluorescence Microscopy
