DNA
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General pages
- Vectors
- Topoisomerase I mediated TA cloning
- Making construction plasmid
- Koch Lab:Publications
- DNA stability
- Nucleic acid structure
- Escherichia coli/Vectors
- Category:User DNA unzipping
- Category:Reagents
- File:Xia, Gamble et al nanoporous nanochannels.gif
- File:Shotgun DNA unzipping.JPG
- P15A
- ColE1
- Primer Tm estimation methods
- DNA separation in different agarose gels
- ApE - A Plasmid Editor (software review)
- PCR inhibitors
- User:Hamid Azizi
- Gene Construction Kit software review
- Category:PCR
- Price comparison PCR reagents
- Price comparison DNA RNA purification kits
- Lane:MagneticPlasmidPrep
- Sequencing services Germany
- MCDS
Protocols
General
- Agarose gel electrophoresis
- DNA ligation
- Yeast DNA Prep
- Yeast Colony PCR
- Ethanol precipitation of small DNA fragments
- Purification of DNA
- Annealing primers
- PNK Treatment of DNA Ends
- Miniprep
- Designing primers
- Phosphatase treatment of linearized vector
- Colony PCR
- Site-directed mutagenesis
- Restriction digest
- Phenol/chloroform extraction
- Sequencing DNA
- PCR
- Ethanol precipitation of nucleic acids
- 'Round-the-horn site-directed mutagenesis
- Library Generation
- Denaturing acrylamide gel purification of nucleic acids
- Resuspension of primers
- Digital Tools & Resources
- Miniprep/Qiagen kit
- Miniprep/Kit-free high-throughput protocol
- BLAST
- One step 'miniprep' method for the isolation of plasmid DNA
- Cloning
- DNA Synthesis from Oligos
- PCR Overlap Extension
- Miniprep/GET buffer
- Electrophoretic mobility shift assay
- Centrifugal filtration/Nucleic acids
- Plasmapper
- Affymetrix DNA labelling for gene expression arrays
- Annealing and primer extension
- Filamentous fungi genomic DNA isolation
- TempliPhi
- Quantitating nucleic acids
- Maxiprep of plasmid DNA from E. coli
- PCR techniques
- Real-time PCR
- QRT-PCR
- Anneal Extend PCR Protocol - for linking 2 pieces of DNA
- Streptavidin purification of DNA fragments
- Mouse tissue lysis for genotyping
- DNA Precipitation
- Q-PCR
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- File:Low tech flow cell.JPG
- File:Low tech flow cell changing buffer with wick.JPG
- Cloning and sequencing
- DNA extraction from tissue
- Engineering BioBrick vectors from BioBrick parts/DNA ligation
- Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
- Engineering BioBrick vectors from BioBrick parts/Restriction digest
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- DNA extraction - Salting Out
- Arabidopsis gDNA isolation
- Assembly pcr
- Cloning Checklist
- File:Unzipping construct.jpg
- Cell and tissue lysis hub
- Bioanalyzer
- Size selective DNA precipitation
- SB
- In-fusion biobrick assembly
- DNA blot (Southern)
- Polyacrylamide gel electrophoresis
- DNA Seq Prep
- Cloning Protocol
- DNA Quantification
- Rapid plant gDNA extraction
- Wwwblast
- Amplified insert assembly
- Miniprep/TENS miniprep
- SPRI bead mix
- Quantifying sequence libraries with qPCR
- Pooling libraries for sequencing
Lab-specific protocols
- MIT:Sequencing BioBrick DNA
- Endy:Templiphi
- Knight:Micropure EZ and Microcon purification
- Knight:Annealing and primer extension with Klenow polymerase
- Knight:DNA ligation using NEB Quick Ligation Kit
- Knight:Colony PCR
- Knight:Restriction Digest
- Knight:Site-directed mutagenesis/Single site
- Silver: PCR
- Knight:Agarose gel electrophoresis
- Knight:TOPO TA cloning
- Endy:Double stranding oligo libraries
- Knight:Sequencing DNA
- Knight:Annealing and primer extension with Taq polymerase
- Knight:Reconstituting primers
- Kafatos:Minipreps
- "Core Sample" PCR: Amplifying DNA from agarose gels
- Knight:Electrophoretic mobility shift assay
- Freimoser:Protocols:FastYeastTransformation
- Jamesh008:consensus DNA ligation protocol
- Lory:Quick transformant screen
- Knight:Maxiprep
- Knight:PhoenIX Maxiprep Kit
- Seth:Quick Transformant Screen
- Knight:TempliPhi
- Sauer:Stitching Genes by PCR
- Koch Lab:Protocols/Dig-bio PCR
- Koch Lab:Protocols/Dig-bio PCR/pRL574 4411 PCR
- Streptomyces:Protocols/DNA Digest
- Etchevers:ChIP francais
- Koch Lab:Protocols/Loading rate clamp
- Knight:Site-directed mutagenesis/Multi site
- Knight:Miniprep low copy plasmids
- Alm:Agarose gel electrophoresis
- Koch Lab:Protocols/Microsphere-DNA tethering
- Knight:DNA ligation using T4 DNA ligase
- Butlin:Unix for Bioinformatics - advanced tutorial
- Butlin:Unix for Bioinformatics - basic tutorial
- NanoBio: PCR
- Sacks:RAD-seq
- Koch Lab:Protocols/Oligonucleotide Annealing/Duplexes
- Koch Lab:Protocols/Oligonucleotide Annealing/Hairpins
- Koch Lab:Protocols/Unzipping constructs
- Koch Lab:Protocols/Fork unzipping constructs
- Koch Lab:Protocols/Unzipping constructs/17mer
- Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment
- Koch Lab:Protocols/Unzipping constructs/17mer/Adapter duplex
- Koch Lab:Protocols/Unzipping constructs/17mer/Downstream unzipping segment
- Duffy:LIC Cloning
- Koch Lab:Protocols/Microsphere-DNA tethering/Glass, dig, biotin, microsphere, 4kb DNA/Cleaning glass
- Weiss Lab:DNA labeling
- Jasieniuk:Converting codominant to dominant data
- Prbbbb:vector pcr
- DIYbio:Notebook/Keiki Gels
- Prbbbb:fusion assembly v1
- Prbbbb:fusion biobrick construction v1
- PrbbBB:colony pcr v1
- Prbbbb:dna measurement on plate v1
- Richard Lab:Agarose Gel Electrophoresis
- Restriction Digest: Partial
- Richard Lab:Restriction Digest
- Erman's Lab:DNA Miniprep with Alkaline Lysis
- Kafatos:Making 1kb+ Ladder Stock
- PrbbBB:Oligo Annealing
- Richard Lab:Amplified insert assembly
- Muratore:Protocols/PCR/Basic
- Muratore:Protocols/Digest/NheI
- Muratore:Protocols/PCR/Quikchange
- Muratore:Protocols/Digest/SapI
- Muratore:Protocols/DNA purification/Miniprep
- Muratore:Protocols/Transformation
- Shreffler:DNA Cleanup
- Corum:PCR
- Corum:digest
- Corum:T4 Ligation
- Corum:PCR Purification
- Corum:Gel Purification
- Corum:DNA Digestion
- Corum:DNA Hybridization
- Corum:Miniprep
- Corum:Protocols
- Corum:DNA Sequencing
- Corum:DNA Quantification
- Corum:Digestion
- Corum:Whole Plasmid PCR
- Milo:No background cloning protocol
- Gill:Rapid bacterial DNA prep
- Brennan:Miniprep for vaccinia virus DNA isolation
Materials
- Acridine Orange
- Reverse Transcriptase
- Phusion
- Terminal Transferase
- Kafatos:DNA 1kbplus ladder
- DNA polymerase
- Pfu
- Thermostable DNA ligase
- DNA ligase
- Cell and tissue lysis hub
- T4 DNA Polymerase
- SB
- Bromodeoxyuridine (BrdU)
- PTXB1
- Pfx50
- SPRI bead mix
- 3D printing models of biomolecules
Resources
See Web resources#DNA and Web resources#DNA 2
