IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System

Super Parts	This is the whole system
Actual Part
Sub Parts	Prey Construct	Predator Construct

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Motivations

• Oscillators are already a fundamental building block in traditional engineering applications
• We wish to build a synthetic biological oscillator that can become a fundamental building block in future synthetic biological applications
• Previous oscillators have been attempted (such as the Repressilator by Elowitz and Leibler in 2000) but they all generally shared the following problems:

• Noise
• Instability
• Inflexible (i.e. lack of control over amplitude and frequency)

• Based on these past limitations, we determined the specifications for our oscillator:

• The oscillations should remain stable for greater than 10 cycles
• It should have a high signal to noise ratio
• It should be flexible (we should be able to control amplitude and frequency)
• It should have connectivity (should be able to be integrated into another system)

Part interface

(describe your black-box approach, define properties of inputs/outputs)

INPUTS OUTPUTS Prey molecule Prey molecule

(don't forget to insert a relevant image of your black box.)

Expected behavior and performances

System level specifications

Properties	Comments
Range	?
Expected output
Latency	?
Robustness	Oscillations should be stable for greater then 10 cycles
Variability	There should be a high signal to noise ratio

Open issues

• list known issues