IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation: Difference between revisions
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!style="background:#D9F2ED"|<center><bbpart>J37023</bbpart><br>(<bbpart>"PCR'd"</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center> || || || | !style="background:#D9F2ED"|<center><bbpart>J37023</bbpart><br>(<bbpart>"PCR'd"</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:J37023 logo.png]]</center> || || || | ||
|- | |- | ||
!style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart> | !style="background:#D9F2ED"|<center><bbpart>J37024</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:J37023 logo.png]]</center> || || | ||
|- | |- | ||
!style="background:#D9F2ED"|<center><bbpart>J37025</bbpart>=<br>(<bbpart> | !style="background:#D9F2ED"|<center><bbpart>J37025</bbpart>=<br>(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)</center>|| || !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:J37024 logo.png]]</center> || | ||
|- | |- | ||
!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center> || || || | !style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center> || || || | ||
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!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]]</center> || || || | !style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]]</center> || || || | ||
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!style="background:#D9F2ED"|<center><bbpart>J37031</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)</center>|| || !!style="background:#CCEBAD" | | !style="background:#D9F2ED"|<center><bbpart>J37031</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)</center>|| || !!style="background:#CCEBAD" |[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:Plus sign.png|20px]][[Image:B0014 logo.png|50px]] | ||
|- | |- | ||
!style="background:#D9F2ED"|<center><bbpart>J37018</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)</center>|| || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:Plus sign.png|20px]][[Image:R0062 logo.png|30px]]</center> | !style="background:#D9F2ED"|<center><bbpart>J37018</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)</center>|| || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:Plus sign.png|20px]][[Image:R0062 logo.png|30px]]</center> | ||
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!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center> | !style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center> | ||
|} | |} | ||
==Quality control== | ==Quality control== | ||
Revision as of 08:22, 29 October 2006
| Super Parts | Not applicable | |
|---|---|---|
| Actual Part | 
| |
| Sub Parts | Prey Construct | Predator Construct |
Ligation strategy
Ligating the Prey Construct:
| For | Stage 1 | Stage 2 |
|---|---|---|
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>) |
   | |
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>) |
 |
Ligating the Polycystronic Predator Construct:
| For | Stage 1 | Stage 2 | Stage 3 | Stage 4 |
|---|---|---|---|---|
(<bbpart>"PCR'd"</bbpart>) |
 |
|||
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>) |
 |
|||
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>) |
 |
|||
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>) |
 |
|||
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>) |
 |
|||
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>) |
 |
Ligating the Monocystronic Predator Construct:
| For | Stage 1 | Stage 2 | Stage 3 | Stage 4 | Stage 5 |
|---|---|---|---|---|---|
(<bbpart>"PCR'd"</bbpart>) |
|
||||
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>) |
|
||||
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>) |
|
||||
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>) |
|
||||
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>) |
|
||||
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>) |
| ||||
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>) |
| ||||
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>) |
|
Quality control
| Part | Logo | Built | Sequenced | Primers Used for Sequencing |
|---|---|---|---|---|
 |
No | N/A | N/A | |
 |
Yes | Yes | Forward pSB1(AK3/A3/A2)
Sequence: TAT TGT CTC ATG AGC GG | |
 |
Yes | Yes | ? | |
|
No | N/A | N/A | |
|
No | N/A | N/A | |
 |
No | N/A | N/A | |
 |
Yes | Not sequenced | N/A | |
 |
No | N/A | N/A | |
|
No | N/A | N/A |
WetLab work entries
- The prey has been constructed and tested but is waiting parameter extraction.
- The predator construct has not been ligated.
- Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
- We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.
- FV:To finish page:
- Need sequencing results for J37023 and any intermediates
- Also need sequencing for B0015 and registry AiiA (from well 5M). A short report on what was wrong with these parts would also be helpful.
