IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation: Difference between revisions
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!height="25pt" width="80pt"|Sub Parts | !height="25pt" width="80pt"|Sub Parts | ||
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey_Construct| Prey | ! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey_Construct| Prey Molecule Generator]] | ||
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator_Construct| Predator | ! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator_Construct| Predator Molecule Generator]] | ||
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<div class="tabs-blue"> | <div class="tabs-blue"> |
Revision as of 11:26, 29 October 2006
Super Parts | Not applicable | |
---|---|---|
Actual Part | ||
Sub Parts | Prey Molecule Generator | Predator Molecule Generator |
Ligation strategy
Ligating the Prey Construct:
For | Stage 1 | Stage 2 |
---|---|---|
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>) |
||
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>) |
Ligating the Polycystronic Predator Construct:
Ligating the Monocystronic Predator Construct:
Quality control
Part | Logo | Built | Sequenced | Primers Used for Sequencing |
---|---|---|---|---|
Yes | ? | ? | ||
Yes | Yes | Forward pSB1(AK3/A3/A2)
Sequence: TAT TGT CTC ATG AGC GG | ||
No | N/A | N/A | ||
Yes | ? | ? | ||
Yes | ? | ? | ||
File:J37033 part.png | Yes | ? | ? |
WetLab work entries
- The prey has been constructed and tested but is waiting parameter extraction.
- The predator construct has not been ligated.
- Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
- We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.
- FV:To finish page:
- Need sequencing results for J37023 and any intermediates
- Also need sequencing for B0015 and registry AiiA (from well 5M). A short report on what was wrong with these parts would also be helpful.