IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation

Super Parts	Not applicable
Actual Part
Sub Parts	Prey Molecule Generator	Predator Molecule Generator

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Ligation strategy

• Ligations for the prey
• Ligations for the predator

• More information on ligations can be found on the Ligation Page.

Quality control

• Quality control for the prey part
• Quality control for the predator part

Implementation

• The oscillator will be tested in a chemostat. This equipment works to:

• Keep the cells in the exponential growth phase
• Keep cell populations constant, ensuring stable oscillations and a high signal to noise ratio
• Vary amplitude and frequency of oscillations by controlling wash-out by the chemostat

WetLab work entries

• The prey has been constructed and tested but is waiting parameter extraction.
• The predator construct has not been ligated.

• Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
• We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.

• FV:To finish page:

• Need sequencing results for J37023 and any intermediates
• Also need sequencing for B0015 and registry AiiA (from well 5M). A short report on what was wrong with these parts would also be helpful.