IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/Implementation
From OpenWetWare
Super Parts | Not applicable | |
---|---|---|
Actual Part | ||
Sub Parts | Prey Construct | Predator Construct |
Ligation strategy
Ligating the Prey Construct:
For | Stage 1 | Stage 2 |
---|---|---|
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>) |
||
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>) |
Ligating the Polycystronic Predator Construct:
Ligating the Monocystronic Predator Construct:
Quality control
Part | Logo | Built | Sequenced | Primers Used for Sequencing |
---|---|---|---|---|
No | N/A | N/A | ||
Yes | Yes | Forward pSB1(AK3/A3/A2)
Sequence: TAT TGT CTC ATG AGC GG | ||
Yes | Yes | ? | ||
No | N/A | N/A | ||
No | N/A | N/A | ||
No | N/A | N/A | ||
Yes | Not sequenced | N/A | ||
No | N/A | N/A | ||
Yes | ? | ? |
WetLab work entries
- The prey has been constructed and tested but is waiting parameter extraction.
- The predator construct has not been ligated.
- Issues included non-functional registry parts such as the registry Aiia and B0015 stop codon.
- We found a new source of AiiA and created J37023 which is a LVA and Flag tagged Aiia gene.