Maheshri:Internal: Difference between revisions
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=Useful Links= | =Useful Links= | ||
*[http://mrw.interscience.wiley.com/emrw/9780471142720/home/ Current Protocols in Molecular Biology] | *[http://mrw.interscience.wiley.com/emrw/9780471142720/home/ Current Protocols in Molecular Biology] | ||
*[http://dbb.urmc.rochester.edu/labs/sherman_f/StartedYeast.html Getting Started with Yeast] | |||
*[http://www.neb.com/nebecomm/DoubleDigestCalculator.asp NEB Double Digest Finder] | *[http://www.neb.com/nebecomm/DoubleDigestCalculator.asp NEB Double Digest Finder] | ||
*[http://www.microscopyu.com/ Nikon MicroscopyU] | *[http://www.microscopyu.com/ Nikon MicroscopyU] | ||
Revision as of 07:39, 5 September 2009
General Lab Policies
- Guidelines for New Members
- Lab Duties
- Standard Operating Procedures for the Equipment
- Common Area Etiquette
- Locations of Common Items and Reagents
- Ordering
- Computing
Protocols
- Media
- Buffers
- Culturing Yeast
- Non-selective Mating of Yeast
- Freezing Cells for Long-term Storage
- PCR
- Restriction Digestion
- Agarose Gel Electrophoresis
- DNA Ligation
- Making Chemically Competent Bacteria
- Bacterial Transformation
- Yeast Transformation
- Bacterial Plasmid Miniprep
- Yeast Genomic Prep
- Yeast Plasmid Prep
- Ethanol Precipitation of Nucleic Acids
- Yeast Protein Prep
- SDS-PAGE
- Western Blotting
- mRNA FISH
- Micrococcal Nuclease Assay
- RT-PCR
- Fluorescence Microscopy
