Maheshri:Internal: Difference between revisions
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*[[Maheshri:Digestion| Restriction Digestion]] | *[[Maheshri:Digestion| Restriction Digestion]] | ||
*[http://openwetware.org/wiki/Agarose_gel_electrophoresis Agarose gel electrophoresis (OpenWetWare)] | *[http://openwetware.org/wiki/Agarose_gel_electrophoresis Agarose gel electrophoresis (OpenWetWare)] | ||
*[[Maheshri:GelEx| Gal Extraction]] | |||
*[http://openwetware.org/wiki/DNA_Ligation DNA Ligation (OpenWetWare)] | *[http://openwetware.org/wiki/DNA_Ligation DNA Ligation (OpenWetWare)] | ||
*[[Maheshri:Competent| Making Chemically Competent Bacteria]] | *[[Maheshri:Competent| Making Chemically Competent Bacteria]] | ||
Revision as of 21:27, 7 September 2009
General Information
- Guidelines for New Members
- Lab Duties
- Standard Operating Procedures for the Equipment
- Common Area Etiquette
- Locations of Common Items and Reagents
- Enzymes
- Abbreviations
- Equipment
- Computing
Protocols
- Media
- Buffers
- Culturing Yeast
- Non-selective Mating of Yeast
- PCR
- Kinase and Anneal Oligos
- Restriction Digestion
- Agarose gel electrophoresis (OpenWetWare)
- Gal Extraction
- DNA Ligation (OpenWetWare)
- Making Chemically Competent Bacteria
- Making Electro-competent Bacteria
- Bacterial Transformation
- Yeast Transformation
- Bacterial Plasmid Miniprep
- Yeast Transformation by Electroporation
- Yeast Genomic Prep
- Yeast Plasmid Prep
- Ethanol Precipitation of Nucleic Acids
- Yeast Protein Prep
- SDS-PAGE
- Western Blotting
- mRNA FISH
- Micrococcal Nuclease Assay
- RT-PCR
- Fluorescence Microscopy
