User:Alexander.wong/sandbox: Difference between revisions
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=New Layout 2 --[[User:Anthony Lazzaro|Anthony Lazzaro]] 19:31, 1 September 2007 (EDT) Alex's I've done tree and so I'd thought i have a go at front page= | |||
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{| style="width:100%; height:100px" | |||
| width="23%" rowspan=1 | [[Image:iGEM-logo.png|130px]] | |||
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| width="52%"| [[Image:ImperialCollege CentenaryLogo.png|280px]] | |||
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{| cellspacing="5" | |||
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<h3><html> | |||
<img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px"> | |||
</html><font color=red>Breaking News !!</font></h3> | |||
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* '''28 Aug 2007''' - 'Infector Modelling' | |||
* '''15 Aug 2007''' - 'We have vesicles!!' | |||
: '''News Archive link''' | |||
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<img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="INFORMATION" border="0"> | |||
</html>At Base Camp</h3> | |||
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* '''[[IGEM:IMPERIAL/2007/Calendar | Calendar of Events]]''' | |||
* '''[[IGEM:IMPERIAL/2007/Literature | Resources]]''' | |||
* '''[[IGEM:IMPERIAL/2007/Ideas|Idea Discussions]]''' | |||
* '''[[IGEM:IMPERIAL/2007/Admin | Admin]]''' | |||
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<img src="http://www.openwetware.org/images/thumb/0/04/Service_protocols.png/40px-Service_protocols.png" alt="INFORMATION" border="0" width="20px"> | |||
</html>In the Wet Lab</h3> | |||
<hr/> | |||
* '''[[IGEM:IMPERIAL/2007/Projects/Lab Notebook | Lab Notebook]]''' | |||
* '''[[IGEM:IMPERIAL/2007/Notebook/General Protocols | Protocols]]''' | |||
* '''[[IGEM:IMPERIAL/2007/DNA Constructs | DNA Constructs]]''' | |||
* '''Sequencing''' | |||
* '''[[IGEM:IMPERIAL/2007/Projects/Shopping Lists | Shopping List]]''' | |||
<h3><html> | |||
<img src="http://www.openwetware.org/images/3/35/Service_collaboration.png" alt="visitors" width="20px" border="0"> | |||
</html>For our Visitors</h3> | |||
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* [[IGEM:IMPERIAL/2007/Feedback | Feedback / Questions]] | |||
<center> | |||
'''Location of our visitors''' | |||
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<a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" /> | |||
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</center> | |||
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<center> | |||
<h2>Imperial College London iGEM'07 Projects</h2> | |||
<big>'''[[IGEM:IMPERIAL/2007/Projects/In-Veso|Cell Free Systems]]'''</big> | |||
[[Image:IC07_CFS_cartoon.png|350px]]</center> | |||
<br> | |||
{|border="0" width="100%" align="center" | |||
! <big><html> | |||
<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="20px"> | |||
</html>'''[[IGEM:IMPERIAL/2007/Projects/Cell By Date|Cell By Date]] '''</big> !! <big><html> | |||
<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="20px"> | |||
</html>'''[[IGEM:IMPERIAL/2007/Biofilm Detector|Infector Detector]]'''</big> | |||
|}<br> | |||
<h3><html> | |||
<img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px"> | |||
</html>Recent Changes</h3> | |||
<hr/>{{Special:Recentchanges/b=IGEM:IMPERIAL/2007&limit=10}} | |||
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<h3><html> | |||
<img src="http://www.openwetware.org/images/3/39/Icon_groups.png" alt="People"> | |||
</html>The Team</h3> | |||
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The Imperial iGEM team consists of ten undergraduate students working fulltime during summer 2007 on engineering a biological system. In addition, we have a number of graduate student and faculty advisors. | |||
<font size="2">'''Undergraduates'''</font> {{hide|1= | |||
* [[User:James Chappell|James Chappell]] | |||
* [[User:Cheuk Ka Tong|Cheuk Ka Tong]] | |||
* [[User:Lucas_A._Hadjilucas|Lucas Hadjilucas]] | |||
* [[User:Jaroslaw Karcz|Jaroslaw Karcz]] | |||
* [[User:Anthony Lazzaro|Anthony Lazzaro]] | |||
* [[User:Peixuan Pey|Peixuan Pey]] | |||
* [[User:Dirk Van Swaay|Dirk van Swaay]] | |||
* [[User:Maira Tariq|Maira Tariq]] | |||
* [[User:Ben_Yi_Tew|Ben Yi Tew]] | |||
* [[User:alexander.wong|Alexander Wong]] | |||
}} | |||
<font size="2">'''Advisors'''</font> {{hide|1= | |||
* Prof Richard Kitney | |||
* Prof Paul Freemont | |||
* Duo Lu | |||
* Kirsten Jensen | |||
* Matthieu Bultelle | |||
* [[User:Vincent|Vincent Rouilly]] | |||
}} | |||
: '''Acknowledgments''' | |||
<h3><html> | |||
<img src="http://www.openwetware.org/images/4/45/GettingStarted_icon.png" alt="Entertainment" width="20px"> | |||
</html>Entertainment</h3> | |||
<hr/> | |||
* '''[[IGEM:IMPERIAL/2007/Pictures | Pictures]]''' | |||
* '''[[IGEM:IMPERIAL/2007/Whiteboard | The Whiteboard]]''' | |||
* '''[[IGEM:IMPERIAL/2007/Quotes | Infamous Quotes]]''' | |||
* '''[[/Team Name and Logo | Discuss team name and logo]]''' | |||
<br> | |||
'''In partnership with''' | |||
[[Image:ImperialCollegeiGEM2007 Geneart Logo .png |left|200px|]] | |||
[http://www.geneart.com GeneART ] | |||
|} | |||
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=Layout for New iGEM page= | =Layout for New iGEM page= | ||
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Revision as of 16:31, 1 September 2007
New Layout 2 --Anthony Lazzaro 19:31, 1 September 2007 (EDT) Alex's I've done tree and so I'd thought i have a go at front page
|
|
<html> <img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px"> </html>Breaking News !!
<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="INFORMATION" border="0"> </html>At Base Camp<html> <img src="http://www.openwetware.org/images/thumb/0/04/Service_protocols.png/40px-Service_protocols.png" alt="INFORMATION" border="0" width="20px"> </html>In the Wet Lab
<html> <img src="http://www.openwetware.org/images/3/35/Service_collaboration.png" alt="visitors" width="20px" border="0"> </html>For our VisitorsLocation of our visitors <html> <a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" /> </a> </html> |
Imperial College London iGEM'07 Projects
<html> <img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px"> </html>Recent ChangesList of abbreviations:
26 April 2024
25 April 2024 |
<html> <img src="http://www.openwetware.org/images/3/39/Icon_groups.png" alt="People"> </html>The TeamThe Imperial iGEM team consists of ten undergraduate students working fulltime during summer 2007 on engineering a biological system. In addition, we have a number of graduate student and faculty advisors. Undergraduates Advisors
<html> <img src="http://www.openwetware.org/images/4/45/GettingStarted_icon.png" alt="Entertainment" width="20px"> </html>Entertainment
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Layout for New iGEM page
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<html> <img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px"> </html>Breaking News !!
<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="INFORMATION" border="0"> </html>At Base Camp<html> <img src="http://www.openwetware.org/images/thumb/0/04/Service_protocols.png/40px-Service_protocols.png" alt="INFORMATION" border="0" width="20px"> </html>In the Wet Lab
<html> <img src="http://www.openwetware.org/images/3/35/Service_collaboration.png" alt="visitors" width="20px" border="0"> </html>For our VisitorsLocation of our visitors <html> <a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" /> </a> </html> |
Imperial College London iGEM'07 Projects
<html> <img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px"> </html>Recent ChangesList of abbreviations:
26 April 2024
25 April 2024 |
<html> <img src="http://www.openwetware.org/images/3/39/Icon_groups.png" alt="People"> </html>The TeamThe Imperial iGEM team consists of ten undergraduate students working fulltime during summer 2007 on engineering a biological system. In addition, we have a number of graduate student and faculty advisors. Undergraduates Advisors
<html> <img src="http://www.openwetware.org/images/4/45/GettingStarted_icon.png" alt="Entertainment" width="20px"> </html>Entertainment
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Questions to ask the fluorometer Engineer on Friday, Aug 3
See if our plate reader has the ability to measure what you want to measure.
- What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
- Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
- Temperature-regulation?
- How do we subtract background values from data and convert from absorbance to OD600?
Talk about using the plate reader and creating protocols.
- Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
- Any manufacturer protocols for plate reader usage?
- Well plate rows and columns? How is data arranged sequentially with the wells?
- What kind of plates you need to order and use for our experiments?
- Lamp energy and Noise signal ratios??
Talk about processing data
- Export format?
- How to export data from fluorometer?
- Built-in data analysis tools?
- Technical Support.
Experiment 1: Fluorescence vs extracellular [GFP] molecules
Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.
Materials Required
Equipment
Reagents
Protocol
Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]
Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].
Materials Required
Equipment
Reagents
Protocol
Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]
Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].
