User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/02: Difference between revisions
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# Spread 100 μL of cells on LB/agar plate. | # Spread 100 μL of cells on LB/agar plate. | ||
# Store plate (inverted) in 37°C oven overnight. | # Store plate (inverted) in 37°C oven overnight. | ||
<b>Gel II</b> | |||
==Description== | |||
# Make a 1% agarose gel by adding 0.25 g of 0.01 g/mL of agarose to 25 mL of 1x tris base, acetic acid, and ethylenediaminetetraacetic acid (TAE) buffer and heating the mixture in the microwave for 40 s. | |||
# Mix 2 μL of 6x gel loading dye with 10 μL of the DNA sample. | |||
# Prepare a ladder containing DNA, glycerol, and gel loading dye. | |||
# Load DNA solutions and run the gel at 80 V for 40 min. | |||
# Remove gel and place in TAE and ethidium bromide (EtBr) solution, mix for 15 min. | |||
# Rinse gel with TAE for 5 min. | |||
==Data== | ==Data== |
Revision as of 09:56, 2 November 2011
AuNP VIII, DNA Transformation II, Gel II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAuNP VIII To synthesize AuNP using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y
To transform DNA coding for mutant GFP (mutated on 09/20/11) into Novablue cells.
To determine whether or not the PCR performed on 11/01/11 of the DNA coding for a mutant GFP was successful using agarose gel electrophoresis.
DescriptionAuNP VIII
Description
DataNotesOrder of DNA gel: Ladder|Tamra 1|Tamra 2|Group 1|Group 2|Group 3|Group 4|Group 5
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