User:Elaine Marie Robbins/Notebook/CHEM-496/2012/03/06

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Au/BSA Mole Ratios Main project page
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To determine the effect of the mole ratio of Au/BSA on AuNP synthesis.


Effect of Au/BSA Ratio

  1. Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4:
    • 90 Au/BSA
    • 110 Au/BSA
    • 130 Au/BSA
    • 150 Au/BSA
  2. Dilute to 1 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3.5 hr.
  5. Centrifuge solutions for 10 min at 13200 rpm.
  6. Take UV-vis data of supernatant.

Effect of Tris Buffer on Protein Fibers

  1. Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12.
  2. Take UV-vis absorption data.
  3. Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12.


Effect of Au/BSA Ratio

  • 90 Au/BSA Ratio
Image:90 ratio.JPG
  • 110 Au/BSA Ratio
Image:110 ratio.JPG
  • 130 Au/BSA Ratio
Image:130 ratio.JPG
  • 150 Au/BSA Ratio
Image:150 ratio.JPG
  • Graph of absorbance vs wavelength for each Au/BSA ratio:
Image:Avsorbance vs wavelength (magnified).png

The transition from AuNP solution to AuNP/BSA fibers occurs between 130 Au/BSA and 150 Au/BSA.

Effect of Tris Buffer on Protein Fibers

  • Graphs of absorbance vs wavelength over time of the AuNP fibers and 50 mM tris (pH 10.1) solution made on 02/15/12:
Image:Absorbance vs wavelength over time 3-6-12.png
Image:Absorbance vs wavelength over time magnified 3-6-12.png


Effect of Au/BSA Ratio

After centrifuge, all four solutions had at least some solid present in the solution. Only the 150 Au/BSA solution had a significant amount of visible fiber formation.

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