User:Jacob Esenther/Notebook/581/2014/09/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Made two new films using ion-exchanged clay | * Made two new films using ion-exchanged clay | ||
* Run AA on arsenic samples | * Run AA on arsenic samples | ||
* Runs for arsenic and mercury samples are temporarily postponed due to technical issues such as defects with the detector and burnt out arsenic lamp. | |||
* Lead (II) acetate · 3 H<sub>2</sub>O was cancelled as well. | |||
==Description== | ==Description== | ||
# Finished film synthesis from 09/19 | # Finished film synthesis from 09/19 | ||
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## Film synthesis can be viewed [[User:Jacob_Esenther/Notebook/581/2014/08/27|here]]. | ## Film synthesis can be viewed [[User:Jacob_Esenther/Notebook/581/2014/08/27|here]]. | ||
## We used our exchanged clay and Melvin's group's exchanged clay | ## We used our exchanged clay and Melvin's group's exchanged clay | ||
### Our ion exchange was with tributylhexadecylphosphonium bromide | ### Our ion exchange was with tributylhexadecylphosphonium bromide (TP) | ||
### Melvin's exchanged with (1-hexadecyl)triphenylphosphonium bromide. | ### Melvin's exchanged with (1-hexadecyl)triphenylphosphonium bromide (TBHD) | ||
# Suspension of Films in Rhodamine 6G | |||
## The arsenic, mercury, and lead were replaced with rhodamine 6G. | |||
## Stock dilutions of 159 μM rhodamine 6G into 1, 10, and 20 μM were prepared by James, Michael, and Eleni. | |||
## The following calculations for the dilution of rhodamine 6G are as follows, taken from Michael Bible's [[User:Michael S. Bible/Notebook/581/2014/09/24|entry:]] | |||
==Data== | ==Data== | ||
* Ion-Exchanged Films | * Ion-Exchanged Films | ||
** Film 1: 1.00888g PVA + 0. | ** Film 1: 1.00888g PVA + 0.11630g TBHD Clay | ||
** Film 2: 1.00123g PVA + 0. | ** Film 2: 1.00123g PVA + 0.11024g other ion exchanged clay | ||
* Preparation of solutions | |||
* The three concentrations of R6G were created from a stock solution of 159 μM R6G. | |||
**1μM R6G - 0.629 mL of stock into 100mL - prepared by Eleni | |||
**10μM R6G - 6.289 mL of stock into 100mL - prepared by Michael | |||
**20μM R6G - 12.578mL of stock into 100mL - prepared by James | |||
* Calculations | |||
V<sub>1</sub>*C<sub>1</sub> = V<sub>2</sub>C<sub>2</sub> | |||
V<sub>1</sub> = V<sub>2</sub>C<sub>2</sub>/C<sub>1</sub> | |||
V<sub>stock</sub> = (100 mL)(1μM)/159μM) = 0.629 mL | |||
V<sub>stock</sub> = (100 mL)(10μM)/159μM) = 6.289 mL | |||
V<sub>stock</sub> = (100 mL)(20μM)/159μM) = 12.578 mL | |||
* Vial Data | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''R6G (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''Empty Vial (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Vial + Film (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Film (g)''' | |||
|- | |||
| PVA||1||||13.04914||13.17062||0.12148 | |||
|- | |||
| ||10||||13.11216||13.27025||0.15809 | |||
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| ||20||||13.08356||13.24444||0.16088 | |||
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|- | |||
| PVA-Clay||1||||13.14655||13.2752||0.12865 | |||
|- | |||
| ||10||||13.11615||13.25209||0.13594 | |||
|- | |||
| ||20||||13.10316||13.2905||0.18734 | |||
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|} | |||
==Notes== | ==Notes== |
Latest revision as of 00:20, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||||||||
Objective
Description
Data
V1*C1 = V2C2 V1 = V2C2/C1 Vstock = (100 mL)(1μM)/159μM) = 0.629 mL Vstock = (100 mL)(10μM)/159μM) = 6.289 mL Vstock = (100 mL)(20μM)/159μM) = 12.578 mL
NotesThis area is for any observations or conclusions that you would like to note.
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