User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/24: Difference between revisions

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==Notes==
==Notes==
====RII overlay====
* PKA Co-IP blot was blocked in the isotope lab
* PKA Co-IP blot was blocked in the isotope lab
* Not all membranes were activated again, while they were maintained under moist conditions
==Results==
==Results==
*{{todo| WHAT?}}
*{{todo| WHAT?}}

Revision as of 03:50, 24 August 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

RII Overlay, Pre-IgG 2nd AB, CSE on HEK293, ASMC to S0, Split cells w. Mileh <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • L314E, 10 μM

Materials & Methods

Materials

Method

Western blot Rabbit pre-immune IgG

  • Secondary AB α-Rabbit (1:10.000), 1h @ RT

RII Overlay

Notes

RII overlay

  • PKA Co-IP blot was blocked in the isotope lab
  • Not all membranes were activated again, while they were maintained under moist conditions

Results

  • WHAT?

Discussion

  • DONE Ow..


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